Preparation of magnetic resonance probes using one-pot method for detection of hepatocellular carcinoma

Table 1 The T1 values of the samples

	1	2	3	4	5	Control
	(0.25 mg)	(0.125 mg)	(0.0625 mg)	(0.031 mg)	(0.016 mg)
USPIO-AFP	427.72 ± 98.67	570.35 ± 110.75	994.36 ± 164.68	1535.9 ± 254.45	2658.2 ± 284.99	2783.4
USPIO-GPC3	392.50 ± 93.25	526.85 ± 109.83	923.55 ± 156.32	1341.8 ± 255.78	2718.1 ± 283.32	2916.1
USPIO	331.08 ± 86.73	523.08 ± 121.47	805.85 ± 145.60	1306.3 ± 202.93	2240.9 ± 268.20	2776.4

1-5 represent the sample of 2 mL 1% agar solution containing the iron concentrations of 0.25, 0.125, 0.0625, 0.031 and 0.016 mg/mL in the anti-AFP-USPIO, anti-GPC3-USPIO or USPIO solution, respectively. USPIO: Ultrasmall superparamagnetic iron oxide; USPIO-AFP: USPIO-α-fetoprotein; USPIO-GPC3: USPIO-glypican 3.

Table 2 The T2 values of the samples

	1	2	3	4	5	Control
	(0.25 mg)	(0.125 mg)	(0.0625 mg)	(0.031 mg)	(0.016 mg)
USPIO-AFP		15.18 ± 0.67	15.92 ± 1.21	25.93 ± 1.13	43.48 ± 1.60	66.20
USPIO-GPC3		36.64 ± 0.42	14.98 ± 0.97	21.48 ± 1.20	42.18 ± 1.52	66.50
USPIO		14.83 ± 0.37	13.44 ± 0.85	22.17 ± 1.02	37.3 ± 1.37	67.67

1-5 represent the sample of 2 mL 1% agar solution containing the iron concentrations of 0.25, 0.125, 0.0625, 0.031 and 0.016 mg/mL in the anti-AFP-USPIO, anti-GPC3-USPIO or USPIO solution, respectively. The signal has gone in 1 test tube in given TEs because the concentration of USPIO-AFP, or USPIO-GPC3, or USPIO is higher. USPIO: Ultrasmall superparamagnetic iron oxide; USPIO-AFP: USPIO-α-fetoprotein; USPIO-GPC3: USPIO-glypican 3.