Increased tumor necrosis factor receptor 1 expression in human colorectal adenomas

Table 1 Summary of the specific antibodies used and immunohistochemistry

Protein	Antibody (catalog number)	Dilution	Incubation
TNF-α	Rabbit polyclonal (Abcam, ab6671)	1:100	Overnight
TNF-R1	Rabbit polyclonal (Abcam, ab19139)	1:2000	1 h
TNF-R2	Rabbit polyclonal (Abcam, ab15563)	1:50	30 min
p-JNK	Rabbit polyclonal (CST, #9251)	1:100	Overnight
NF-κB	Rabbit polyclonal (CST, #3034)	1:50	Overnight
Caspase-3	Rabbit polyclonal (CST, #9661)	1:200	Overnight

Abcam (Cambridge, United Kingdom). CST: Cell signaling technology (Beverly, MA, United States).

Table 2 Comparison of the clinical data and measured biochemical parameters in the serum of patients with adenomas and control subjects (mean ± SD)

Parameters	Control (n = 34)	Adenoma (n = 62)	P value
Age (yr)	67.6 ± 15.9	67.7 ± 8.2	NS
Sex (F/M)	14/20	23/39	NS
BMI	23.5 ± 5.0	23.9 ± 3.4	NS
TNF-α (pg/mL)	5.56 ± 1.3	4.83 ± 1.9	NS
sTNF-R1 (ng/mL)	1.57 ± 0.72	3.67 ± 0.86	< 0.001
sTNF-R2 (ng/mL)	2.72 ± 1.15	3.09 ± 0.91	NS

P < 0.05 was denoted significance. NS: Non-significant; TNF-α: Tumor necrosis factor-α; sTNF-R1/2: Soluble tumor necrosis factor receptor 1/2; F/M: Female/male; BMI: Body mass index.

Table 3 Association between the measured biochemical parameters and the number of colorectal adenomas per person

	Control	≤3	≥4	P value
	(n = 34)	(n = 34)	(n = 28)
TNF-α (pg/mL)	5.63 ± 1.3	4.31 ± 1.9	4.95 ± 2.0	NS
sTNF-R1 (ng/mL)	1.57 ± 0.72	3.37 ± 0.93	4.05 ± 0.55	< 0.001
sTNF-R2 (ng/mL)	2.72 ± 1.15	3.13 ± 0.88	3.04 ± 0.95	NS

Values are expressed as the mean ± SD; P < 0.05 denoted significance. NS: Non-significant; TNF-α: Tumor necrosis factor-α; sTNF-R1/2: Soluble tumor necrosis factor receptor 1/2.