Activation of human colon mast cells through proteinase activated receptor-2

Table 1 Effects of tc-LIGRLO, tc-OLRGIL, anti-IgE and cal-cium ionophore A23187 (CI) on tryptase and histamine release from colon mast cells following 30 min incubation period

Concentration of stimulus		Tryptase (ng/mL)	Histamine (ng/mL)
Buffer alone		8.2 ± 0.6	9.5 ± 1.7
tc-LIGRLO	100 µmol/mL	22 ± 3.0a	17 ± 1.3a
tc-OLRGIL	100 µmol/mL	8.4 ± 2.8	10 ± 1.9
Anti-IgE	10 µg/mL	19 ± 4.1a	19 ± 4.8a
CI	1.0 µg/mL	13 ± 0.6a	34 ± 4.3a

The values shown are mean ± SEM for four separate experiments performed in duplicate.

^aP < 0.05 compared with buffer alone group (paired Student’s t test).

Table 2 Inhibition of tc-LIGRLO, SLIGKV, trypsin, anti-IgE or calcium ionophore A23187 (CI) induced tryptase release from colon mast cells by pertussis toxin (1 µg/mL) and metabolic inhibitors

Concentration of stimulus		% inhibition of tryptase release
		Pertussis toxin	Metabolic inhibitors
tc-LIGRLO	100 µmol/mL	85 ± 9.6a	91 ± 9.3a
SLIGKV	100 µmol/mL	91 ± 4.8a	85 ± 9.6a
Trypsin	10 µg/mL	100 ± 0a	91 ± 3.9a
Anti-IgE	10 µg/mL	87 ± 10a	94 ± 3.6a
CI	1.0 µg/mL	84 ± 8.8a	95 ± 4.0a

The values shown are mean ± SEM for four separate experiments performed in duplicate.

^aP < 0.05 compared with the uninhibited control group (paired Student’s t test).

Table 3 Inhibition of tc-LIGRLO, SLIGKV, trypsin, anti-IgE or calcium ionophore A23187 (CI) induced histamine release from colon mast cells by pertussis toxin (1 µg/mL) and metabolic inhibitors

Concentration of stimulus		% inhibition of histamine release
		Pertussis toxin	Metabolic inhibitors
tc-LIGRLO	100 µmol/mL	92 ± 5.4a	98 ± 2.0a
SLIGKV	100 µmol/mL	95 ± 5.9a	92 ± 3.8a
Trypsin	10 µg/mL	84 ± 6.8a	88 ± 8.7a
Anti-IgE	10 µg/mL	84 ± 5.2a	99 ± 1.1a
CI	1.0 µg/mL	97 ± 2.4a	99 ± 0.1a

The values shown are mean ± SEM for four separate experiments performed in duplicate.

^aP < 0.05 compared with the uninhibited control group (paired Student’s t test).