Quantitative detection of common deletion of mitochondrial DNA in hepatocellular carcinoma and hepatocellular nodular hyperplasia

Table 1 TaqMan primers and probes for detection of WT-mtDNA and CD-mtDNA

Target	Amplicon	Oligonucleotide sequence (5’-3’)
Wild Type mtDNA	101 bp	WT1 forward primer (7878-7897) : 5´-AATCAATTGGCGACCAATGG-3´
		WT2 reverse primer (7979-7958) : 5´-CGCCTGGTTCTAGGAATAATGG-3´
		WT probe (7899-7917) : 5’FAM-ACTGAACCTACGAGTACAC-MGB-3’
Common Deletion mtDNA	132 bp	CD1 forward primer (8448-8472) : 5’-TATTAAACACAAACTACCACCTACC-3’
		CD2 reverse primer (13560-13539) : 5’-GGCTCAGGCGTTTGTGTATGAT-3’
		CD probe: (13456-13471) : 5’FAM- ACC ATTGGC AGC CTA G -MGB 3’