Expression of TIMP-1 and TIMP-2 in rats with hepatic fibrosis

Table 1 Sequences of TIMP-1, TIMP-2 and β-actin primers

	Primer	Nucleic acid sections	Position (bp)
TIMP-1	Positive strand	5’-TTCGTGGGGACACCAGAAGTC-3’
	Antisence strand	5’-TATCTGGGACCGCAGGGACTG-3’	482
β-actin	Positive strand	5’-GGAGAAGATGACCCAGATCA-3’
	Antisence strand	5’-GATCTTCATGAGGTAGTCAG-3’	234
TIMP-2	Positive strand	5’-GTTTTGCAATGCAGATGTAG-3’
	Antisence strand	5’-ATGTCGAGAAACTCCTGCTT-3’	540
β-actin	Positive strand	5’-ACCCCCACTGAAAAA-3’
	Antisence strand	5’-ATCTTCAAACCTCCATGATG-3’	120

Table 2 Expression of TIMP-1 and TIMP-2 related antigens in rat liver

Group	n	TIMP-1	TIMP-2
Experimental group	20	482.50 ± 65.00	336.50 ± 48.32
Normal group	10	59.8 ± 20.31	49.86 ± 18.54

P < 0.001.

Table 3 Gene expression of TIMP-1 and TIMP-2 in rat liver

Group	n	TIMP-1/β-actin	TIMP-2/β-actin
Experimental group	20	1.86 ± 0.47	1.06 ± 0.22
Normal group	10	0.36 ± 0.08	0.36 ± 0.08

P < 0.001.