Expression of Helicobacter pylori Hsp60 protein and its immunogenicity

doi:10.3748/wjg.v9.i12.2711

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Dec 15, 2003 (publication date) through Apr 25, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Dec 15, 2003; 9(12): 2711-2714
Published online Dec 15, 2003. doi: 10.3748/wjg.v9.i12.2711

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Figure 3 100 g·L^-1 SDS-PAGE analysis of the fusion protein expressed in BL21(DE3). Lane1: control strain BL21(pET) be-fore induction, Lane2: control strain BL21(pET) after 3 h in-duction with IPTG, Lane3: purified recombinant protein Hsp60, Lane4: sonicated supernatant of BL21(pET-Hsp60) cells after 3 h induction with IPTG, Lane5: BL21(pET-Hsp60) cell periplasm protein after 3 h induction with IPTG, Lane6: BL21 (pET- Hsp60) cells after 5 h induction with IPTG, Lane7: BL21 (pET- Hsp60) cells after 3 h induction with IPTG, Lane8: BL21 (pET-Hsp60) cells before induction, Lane9: molecular weight marker (20,30,43,67,94)×10³

Citation: Bai Y, Li LR, Wang JD, Chen Y, Jin JF, Zhang ZS, Zhou DY, Zhang YL. Expression of Helicobacter pylori Hsp60 protein and its immunogenicity. World J Gastroenterol 2003; 9(12): 2711-2714
URL: https://www.wjgnet.com/1007-9327/full/v9/i12/2711.htm
DOI: https://dx.doi.org/10.3748/wjg.v9.i12.2711