DNA immunization with fusion genes encoding different regions of hepatitis C virus E2 fused to the gene for hepatitis B surface antigen elicits immune responses to both HCV and HBV

Figure 4 IgG subtypes profile of antibodies against HBsAg and HCV E2. Sera collected from mice 6 weeks after boosting were assayed for the IgG1 and IgG2a antibodies against HBsAg and HCV E2. For each group of 5 mice, titers of IgG1 and IgG2a antibodies were determined individually by serial two-fold dilution titration methods using HRP-conjugated rabbit anti-mouse IgG1 and IgG2a (Serotec Co., Oxford, UK) for detection. (as described in Materials and Methods). The arithmetic mean ± standard deviation (SD) (n = 5) is shown. (A) Anti-HBs, (B) Anti-HCV E2.