Basic Research
Copyright ©The Author(s) 2002. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jun 15, 2002; 8(3): 540-545
Published online Jun 15, 2002. doi: 10.3748/WJG.v8.i3.540
Evidences for vagus nerve in maintenance of immune balance and transmission of immune information from gut to brain in STM-infected rats
Xi Wang, Bai-Ren Wang, Xi-Jing Zhang, Zhen Xu, Yu-Qiang Ding, Gong Ju
Xi Wang, Bai-Ren Wang, Zhen Xu, Yu-Qiang Ding, Gong Ju, Institute of Neuroscience, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
Xi-Jing Zhang, Department of Anesthesiology, Xi Jing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
Author contributions: All authors contributed equally to the work.
Supported by National Natural Science Foundation, No. 39830130
Correspondence to: Gong Ju, Institute of Neurosciences, The Fourth Military Medical University, Xi’an, P.R. China. jugong@fmmu.edu.cn
Telephone: +86-29-3374557 Fax: +86-29-3246270
Received: November 2, 2001
Revised: November 15, 2001
Accepted: November 27, 2001
Published online: June 15, 2002

Abstract

AIM: To determine whether Salmonella Typhimurium (STM) in gastrointestinal tract can induce the functional activation of brain, whether the vagus nerve involves in signaling immune information from gastrointestinal tract to brain and how it influences the immune function under natural infection condition.

METHODS: Animal model of gastrointestinal tract infection in the rat was established by an intubation of Salmonella Typhimurium (STM) into stomach to mimic the condition of natural bacteria infection. Subdiagphragmatic vagotomy was performed in some of the animals 28 days before infection. The changes of Fos expression visualized with immunohistochemistry technique in hypothalamic paraventricular nucleus (PVN) and superaoptic nucleus (SON) were counted. Meanwhile, the percentage and the Mean Intensities of Fluorescent (MIFs) of CD4+ and CD8+ T cells in peripheral blood were measured by using flow cytometry (FCM), and the pathological changes in ileum and mesenteric lymph node were observed in HE stained sections.

RESULTS: In bacteria-stimulated groups, inflammatory pathological changes were seen in ileum and mesenteric lymph node. The percentages of CD4+ T cells in peripheral blood were decreased from 42% ± 4.5% to 34% ± 4.9% (P < 0.05) and MIFs of CD8+ T cells were also decreased from 2.9 ± 0.39 to 2.1 ± 0.36 (P < 0.05) with STM stimulation. All of them proved that our STM-infection model was reliable. Fos immunoreactive (Fos-ir) cells in PVN and SON increased significantly with STM stimulation, from 189 ± 41 to 467 ± 62 (P < 0.05) and from 64 ± 21 to 282 ± 47 (P < 0.05) individually, which suggested that STM in gastrointestinal tract induced the functional activation of brain. Subdiagphragmatic vagotomy attenuated Fos expression in PVN and SON induced by STM, from 467 ± 62 to 226 ± 45 (P < 0.05) and from 282 ± 47 to 71 ± 19 (P < 0.05) individually, and restored the decreased percentages of CD4+ T cells induced by STM from 34% ± 4.9% to original level 44% ± 6.0% (P < 0.05). In addition, subdiagphragmatic vagotomy itself also decreased the percentages of CD8+ T cells (from 28% ± 3.0% to 21% ± 5.9%, P < 0.05) and MIFs of CD4+ (from 6.6 ± 0.6 to 4.9 ± 1.0, P < 0.05) and CD8+ T cells (from 2.9 ± 0.39 to1.4 ± 0.34, P < 0.05). Both of them manifested the important role of vagus nerve in transmitting immune information from gut to brain and maintaining the immune balance of the organism.

CONCLUSION: Vagus nerve does involve in transmitting abdominal immune information into the brain in STM infection condition and play an important role in maintenance of the immune balance of the organism.



Citation: Wang X, Wang BR, Zhang XJ, Xu Z, Ding YQ, Ju G. Evidences for vagus nerve in maintenance of immune balance and transmission of immune information from gut to brain in STM-infected rats. World J Gastroenterol 2002; 8(3): 540-545
INTRODUCTION

It has been suggested in recent studies that the vagus nerve, the tenth cranial nerve, might play an important role in transmitting immune information into the brain[1-5]. However, this conclusion is based on the experiments in which cytokines, endotoxins or exotoxins were usually used as immune stimulators through intraperitoneal or intravenous injection. All these immune stimulations, however, are non-natural and the role of vagus in natural infection condition has not been established yet. Salmonella Typhimurium (STM) belongs to the group B of Salmonella. It can infect both human beings and animals through gastrointestinal tract and leads to a local or general infection by inhibiting the host immune system[6]. Thus, in the current experiments we introduced Salmonella Typhimurium (STM) into stomach to mimic the natural bacteria infection in gastrointestinal tract and to reassess the role of vagus in transmission of immune signal by subdiagphragmatic vagotomy. The production of c-fos, an immediately early gene, has been used as a morphological marker of functionally activated brain neurons[7-15]. In the present study we observed the STM-induced Fos expression in hypothalamic paraventricular nucleus (PVN) and superaoptic nucleus (SON) and the effect of vagotomy. We also studied the importance of integrity of vagus nerve in the balance of T cell subpopulations.

MATERIALS AND METHODS
Animals

Adult male Sprague Dawley albino rats (180-210 g, offered by Animal Center, Fourth Medical University) were used. Rats were housed individually in a temperature-controlled room in a natural light/dark cycle, with food and water available freely. The animals were trained for adaptation to handling and gastric intubation before the following procedures started.

Procedures

Subdiagphragmatic vagotomy Rats were anesthetized with pentobarbital sodium (40 mg/kg, i.p.) and subjected to a complete subdiagphragmatic vagotomy (n = 10) or sham operation (n = 10). Briefly, after laparotomy, the two trunks of vagus were identified under an operating microscope. Both trunks were cut off close to the diaphragm. For sham vagotomy, the vagus was similarly exposed but was not cut. After surgery, a recovery period of 28 days was allowed.

Preparation of STM Wild strain of STM (offered by Laboratory of Bacteria, Xijing Hospital, Fourth Medical University) was preserved in freeze-dried powder before use. In order to enhance the pathogenicity of the bacteria, STM were sub-cultured in mice abdomen (Kunming mice offered by Animal Center, Fourth Medical University) for 3 times and then the number of the bacteria was adjusted to 1010/mL for use.

Intubation of STM Rats (n = 20) were divided into 4 groups randomly, 5 for each. Group 1, saline (NS) + sham operation; 2, NS + vagotomy; 3, STM + sham operation; 4, STM + vagotomy. Food was taken away from the rats 24 h prior to intubating STM or saline. After anesthetized with ether, all rats were intubated with 30 g/L NaHCO3 300 μl to neutralize gastric acid. Then the aminals of groups 3 and 4 were gastrically intubated with STM (1010 in saline, 1 mL) and in the others (Groups 1 and 2) 1 mL of saline were given.

Perfusion and Sectioning After intubation for 22 h, all rats were deeply anesthetized with pentobarbital (80 mg/kg) and 1 mL of blood was taken via heart as quickly as possible. The rats were then perfused transcardially with saline 100 mL followed by 4% paraformaldehyde in 0.1M phosphate buffer (PB) 500 mL, pH7.4, at 4 °C. Blood was anti-coagulated with heparin. Brains, part of ileum and mesenteric lymph node were taken out and cryoprotected in 20% sucrose in 0.1 M PB overnight at 4 °C. Frontal sections in 50 μm-thickness were cut through whole brains with a microtome and collected in cold cryoprotectant and stored at -20 °C until immunohistochemistry processing. Serial ileum and mesenteric lymph node sections in 5 μm-thickness were cut with a cyostat and mounted onto slides coated with gelatin and stored at -20 °C until histochemistry processing.

HE staining of ileum and mesenteric lymph node sections Slides of ileum and mesenteric lymph node were immersed successively in dimethylbenzene (10 min × 2), graded ethanol (100% 5 min × 2, 95% 2 min, 80% 2 min, 70% 2 min and distilled water 2 min), Harris hematoxylin (5-10 min) and 10% acid ethanol for several seconds. After rinsed with tap water for 30 min, slides were immersed again successively in distilled water (10-30 min), graded ethanol (70%, 80%, and 95% 2 min for each), 0.5% eosin (5-10 min), 95% ethanol from several seconds to minutes, 100% ethanol (5 min × 2) and dimethylbenzene (10 min × 2). At last, the slides were sealed with gum and observed under a light microscope (Olympus B × 60).

Flow cytometry (FCM) of blood T Cell Blood CD4+ and CD8+ Tlymphocytes were labeled by using indirect immunofluorecent labeling method. First, 80 μl of anti-coagulated blood was incubated with mice anti rat CD4 mAb (1:100, Serotec company) or 15 μl of mice anti rat CD8 mAb (1:100, Serotec company) for 30 min at 4 °C, then with 40 μl of goat anti mice IgG-FITC (1:100, Serotec company) after washing twice with 0.01Mol/L Phosphate-buffered saline (PBS). FCM was used to detect the percentages and the Mean Intensities of Fluorescence (MIFs) of CD4+ and CD8+ T cells.

Immunohistochemistry of Brain Sections ABC immunohisto chemical technique was used to detecte Fos-immunoreactive (Fos-ir) cells in brain. One-in-five of brain sections were incubated with primary antibody raised from rabbit against Fos protein (Sigma Inc.) at a dilution of 1:3000. After incubation at room temperature for 36 h, sections were rinsed with 0.01Mol/L Phosphate-buffered saline (PBS) (10 min × 3) and then incubated with biotinylated secondary antibody against rabbit IgG (Sigma Inc, diluted at 1:500) at room temperature for 4 h. After rinsing with 0.01Mol/L PBS (10 min × 3), sections were incubated with avidin-biotin-horseradish peroxidase (1:500, Sigma Inc.) at room temperature for 2 h. The reaction product was visualized with amine nickel sulfate-enhanced 3,3’-diaminobenzidine (DAB) method. The sections were dehytrated in graded ethanol, cleared with dimethylbenzene, and coverslipped with gum.

Counting of Fos-ir cells Sections of hypothalamus were observed with a light microscope (Olympus BX60). The number of Fos-ir cells was quantified by counting immunostained nuclei in PVN or SON at two consecutive typical sections with an image analysis system (Leica Quantimet 570 C). The number of Fos-positive nuclei in PVN or SON was the group mean ± SE.

Statistical analyses All data were expressed as mean ± SE and were analyzed by one-way ANOVA. Post hoc analysis was done by using the Student-Newman-Keuls (SNK) multiple comparison test. A value of P < 0.05 was considered significant.

RESULTS
HE staining

Inflammation change was seen in ileum and mesenteric lymph node in the rats stimulated with STM. There are numerous bacilli in ileum cavity in the infected rats. The structure of the villus of the infected ileum was destroyed (Figure 1, E2), part of epithelial cells were scaled, and many neutrophil, red blood cell and fibroblast infiltrated into the villus. At the same time, secondary lymphoid folliculi appeared in mesenteric lymph node (Figure 1, E4). Figure 1, E1 and E3 show the normal tissue image of the villus and mesenteric lymph node in saline-treated rat.

Figure 1
Figure 1 In Figures E1 and E3 show the normal structures of the villus and mesenteric lymph node in saline-injected rats; 2 and 4 show the villus and mesenteric lymph node in STM-challenged rats. 5 and 9 show Fos expression in PVN and SON respectively in NS + sham rats; 6 and 10 show Fos expressions in PVN and SON respectively in STM + sham rat; 7 and 11 show Fos expressions in PVN and SON respectively in STM + vagotomy rat; 8 and 12 show Fos expressions in PVN and SON respectively in NS + vagotomy rat. × 50
Figure 2
Figure 2 A: Percentages of blood CD4+ T cells. aP < 0.05 vs. STM + sham, bP < 0.05 vs NS + sham; B: The Mean Intensities of Fluorescence (MIFs) of blood CD4+ T cells. bP < 0.05 vs NS + sham; C: Percentages of blood CD8+ T cells. aP < 0.05 vs STM + sham, bP < 0.05 vs NS + sham; D: The Mean Intensities of Fluorescence (MIFs) of blood CD8+ T cells. aP < 0.05 vs STM + sham; bP < 0.05 vs NS + sham.
FCM

Table 1 shows the percentages and MIFs of CD4+and CD8+ T cells in every group.

Table 1 Percentages (%) and MIF of CD8+ and CD4+ T cells (-x±s).
NS + shamNS + vagotomySTM + shamSTM + vagotomy
CD442 ± 4.546 ± 4.634 ± 4.9b44 ± 6.0a
CD4 MIF6.6 ± 0.64.9 ± 1.0b6.8 ± 1.16.1 ± 1.0
CD828 ± 3.021 ± 5.9b23 ± 2.017 ± 5.8a
CD8 MIF2.9 ± 0.391.4 ± 0.34b2.1 ± 0.36b1.1 ± 0.06a

Figure 2A shows that subdiagphragmatic vagotomy itself in normal animals had no evident effect on the percentages of CD4+ T cells, but the stimulation of STM itself in sham-operated animals decreased the percentages of CD4+ T cells from 42% ± 4.5% to 34% ± 4.9% (P < 0.05) and after subdiagphragmatic vagotomy the decreased percentages of CD4+ T cells in STM stimulated rats restored from 34% ± 4.9% to 44% ± 6.0%, the level of non-STM stimulated rats (P < 0.05).

Figure 2B shows that subdiagphragmatic vagotomy itself in NS+operation animals decreased MIFs of CD4+ T cells from 6.6 ± 0.6 to 4.9 ± 1.0 (P < 0.05), indicating the inhibition of subdiagphragmatic vagotomy to CD4+ T cells.

Figure 2C and Figure 2D show that subdiagphragmatic vagotomy itself in normal rats decreased the percentages of CD8+ T cells (from 28% ± 3.0% to 21% ± 5.9%, P < 0.05) as well as MIFs of CD8+ T cells (from 2.9 ± 0.39 to 1.4 ± 0.34, P < 0.05). STM stimulation itself in sham-operated rats also depressed the percentages of CD8+ T cells (from 28% ± 3.0% to 21% ± 5.9%, P > 0.05) and MIFs of CD8+ T cells (from 2.9 ± 0.39 to 2.1 ± 0.36, P < 0.05). Subdiagphragmatic vagotomy in STM-challenged rats aggravated the inhibition of STM to the percentages of CD8+ T cells (from 23% ± 2.0% to 17% ± 5.8%, P < 0.05) and MIFs of CD8+ T cells (from 2.1 ± 0.36 to 1.1 ± 0.06, P < 0.05).

Immunohistochemistry

Table 2 shows the number of Fos-ir cells in PVN and SON in each group. The numbers of Fos-ir cells in PVN and SON of STM + sham-operated rats increased significantly compared with that of NS + sham from 189 ± 41 to 467 ± 62 (P < 0.05) and from 64 ± 21 to 282 ± 47 (P < 0.05) individually (Figure 1, E5, E6, E9, E10). The positive neuron distributed in both magnocellular and parvocellular portions of PVN as well as dorsal and ventral parts of SON. Fos expressions were attenuated in PVN and SON in the rats of STM+vagotomy group compared with that of STM + sham group from 467 ± 62 to 226 ± 45 (P < 0.05) and from 282 ± 47 to 71 ± 19 (P < 0.05) individually (Figure 1, E6, E7, E10, E11), but it was still higher than that of saline-treated animal (189 ± 41 and 64 ± 21 individually). There was no significant changes of Fos expression in NS + vagotomy rats compared with NS + sham rats (Figure 1, E5, E8, E9, E12).

Table 2 Numbers of Fos-ir Cells in PVN and SON (-x±s).
NS + shamNS + vagotomySTM + shamSTM + vagotomy
PVN189 ± 41131 ± 38467 ± 62a226 ± 45b
SON64 ± 2149 ± 22282 ± 47a71 ± 19b
DISCUSSION

More and more evidences have shown that there is a complicated bidirectional inter-relationship between nervous system and immune system[4,16-23]. Immune signals produced during antigen challenge can be transmitted into central nervous system (CNS) and influence the function of the latter. In turn, CNS can modulate the activity of immune system. However, it is still an unsolved problem up to now how the immune signals are transmitted into CNS. Two of hypothesises have been proposed[1-4]: one is through humoral route and the other, via neural pathway. Among the neural pathways the vagus nerve in transferring peripheral immune signals into CNS has been paid more attention to[1-5,24,25]. A large amount of evidences indicate that vagus plays an important role in surveying the peripheral immune information into CNS. For example, subdiagphragmatic vagotomy inhibits a series of brain-mediated responses to peripheral administration of lipopolysaccharide (LPS), IL-1β or TNF-β, such as induction of IL-1β mRNA within mice brain[26,27], activation of hypothalamic corticotropin-releasing hormone neurons and ACTH secretion[28,29], LPS-induced fever in guinea pigs[30], Fos immunoreactivity in primary afferent neurons of the vagus[31], the inhibition of social exploration[32], a monophasic fever[33], the hyperalgesia[34,35] etc. Administration of IL-1β in hepatic portal vein induced afferent discharges of hepatic branch of vagus, but the discharges disappeared in vagotomy rats[36]. Nucleus tractus solitarius lesions attenuated the first fever peak induced by intraperitoneal injection of IL-1β[37].All of the above mentioned experiments indicate that intact vagus is necessary for transmitting the immune information from periphery, especially from peritoneal cavity, to the brain. According to the anatomical structure of vagus, the abdominal organs such as liver, stomach, intestines, lymph node, etc. are innervated mostly by subdiagphragmatic vagus and the vagus contains important visceral sensory afferent fibers from abdominal organs[38,39]. Thus, we conjecture that subdiagphragmatic vagus may play an important role in transmitting the abdominal immune information into the brain and is important in maintaining immune balance.

All the immune challenges used in previous studies were bacterial toxins such as LPS or immune cytokines injected intraperitoneally or intravenously. In this experiment we established a rat model of gastrointestinal tract infection by STM intubation to mimic the natural infection and a subdiagphragmatic vagotomy was performed to further observe the role of vagus in immune signal transmission. According to aetiology, STM can invade intestinal mucosa and largely reproduce, and then further spread into the drained mesenteric lymph nodes and disseminate via the bloodstream[40]. STM is an intracellular Gram-negtive bacterial pathogen that infects both phagocytic and non-phagocytic cells[6,40-43]. It can inhibit the host immune system and cause a range of diseases including enteric fever and gastroenteritis[6]. It has been reported that the depletion of either CD4+ or CD8+ T cells by STM impairs their ability to transfer protective immunity to virulent S. typhimurium[6]. These studies indicate that CD4+ and CD8+ T cells act synergistically to control infection with virulent S. typhimurium[6,44,45]. In our experiment the villus of the infected ileum was destroyed, part of epithelial cells scaled, and the number of neutrophils, red blood cells as well as fibroblasts increased in the villus. At the same time, secondary lymphoid folliculus stimulated with STM emerged in mesenteric lymph nodes. The percentages of CD4+ and CD8+ T cells and MIFs of CD8+ T cells of peripheral blood were all inhibited, which was consistent with the previous reports. These changes induced by STM suggest that our STM infection model was reliable.

The result showed that in NS + sham rats Fos proteins expressed in a few of PVN and SON neurons, which suggests that in normal condition some PVN and SON neurons are active, and may is related to the modulation of routine metabolic activities. After being stimulated with STM the number of Fos-ir cells significantly increased in PVN and SON. It indicated that these cells were activated by STM-challenge. It is well known that CNS, especially hypothalamus, involves in modulation of acute immune reaction[46]. PVN and SON, which are two most important nuclei in hypothalamus related to autonomic function, are mainly composed of three kinds of neurons neurochemically: oxytocinergic, vasopressinergic and CRH neurons[46]. All of these three kinds of neurons can involve in neuroimmunomodulation[46]. Yang et al[46] reported that, as the neuroimmunomodulation integrating center, hypothalamic PVN modulates the immune function through three pathways: The first is CRH-ACTH-adrenal cortex axis, the second is oxytocin neuroendocrine pathway, and the third is PVN-spinal cord sympathetic preganglionic projection. Although we can’t determine which kind of neurons were activated in this experiment since we did not apply double-labeling technique to identify them, we proposed from the observation of distribution of Fos positive neurons in the subnuclei of PVN and SON that, maybe, all these three kind neurons were activated.

But, how the immune signals are transmitted into the brain is an important and unsolved question. Is it through vagus or humoral pathway, or both of them? What we focused on in the present study was the role of vagus in the sensation and transmission of immune signals to brain. So, we severed subdiagphragmatic vagus to observe whether the Fos expressions in PVN and SON induced by STM infection and the T cell subpopulation were influenced. After subdiagphragmatic vagotomy, Fos expressions in PVN and SON were attenuated. At the same time we found that the decreased percentage of CD4+ T cells in STM-infected rats restored after subdiagphragmatic vagotomy. These results indicate the importance of intact subdiagphragmatic vagus in signaling immune information from abdominal organs to CNS. We tend to conclude from our results that subdiagphragmatic vagus does play a role in transferring immune information into brain during the abdominal inflammatory phase.

However, the detailed mechanism about how vagus nerve senses the immune stimulation and transfers it into electric signal is still not fully understood. It is known that macrophages, dendritic cells, and other immune cells detect and present antigens and respond by releasing proinflammatory mediators, such as IL-1β, IL-6 and TNF-α[23,47,48]. Goehler et al[47] found that between the fibers of abdominal vagus there exist immune cells which can produce IL-1β. IL-1β acts to both coordinating the peripheral immune response and signaling the CNS[49]. The globe cells of vagus paraganglia near liver hilus could be stained by biotinylated IL-1 receptor antagonist[50] and by anti rat IL-1 receptor type I antibody[51], which suggested the possibility for vagus to sense the local IL-1. We[51] and others[52] also have reported that the primary sensory neurons in nodose ganglia of vagus contain IL-1 receptor protein and mRNA, which indicates that vagus nerve probably can sense IL-1 directly.

It is necessary to point out that vagus is definitely not the only route for immune signal getting into the brain, since it is found in the present study that although the number of STM stimulation-induced Fos expressed neurons in hypothalamus is attenuated after vagotomy, the number is still higher than that in control. So the humoral pathways or other nerves may also involve in the immune signals transmission in some degree, which still needs further study.

Our results also showed that subdiagphragmatic vagotomy itself decreased the percentages of CD8+ T cells and MIFs of CD4+ and CD8+ T cells, which indicated the importance of intact vagus in maintaining the host immune balance. This is also accordant with our previous study[53]. As we know that CD4+ and CD8+ T cells are necessary in clearing STM[6,54,55]. Vagotomy inhibits the subpopulation of T cells, which is a disadvantage to STM clearance and only aggravate the inhibition to CD4+ and CD8+ T cells induced by STM. How does the vagus influence the phenotype of lymphatic cells? Vagus contains both afferent and efferent fibers innervating abdomen. The former can transmit abdominal information into CNS and the latter innervates some immune organs or immune cells, such as abdominal lymph node. When we cut off subdiagphragmatic vagotomy, on the one hand, the abdominal immune information can’t be transmitted into the brain; on the other hand, the brain can’t influence the abdominal immune organizations via vagus. We suppose that this is probably the answer.

In summary, subdiagphragmatic vagus is able to signal immune information from abdomen into the brain and intact vagus is necessary in maintaining the host immune balance.

Footnotes

Edited by Hu DK

References
1.  Maier SF, Goehler LE, Fleshner M, Watkins LR. The role of the vagus nerve in cytokine-to-brain communication. Ann N Y Acad Sci. 1998;840:289-300.  [PubMed]  [DOI]
2.  Goehler LE, Gaykema RP, Hansen MK, Anderson K, Maier SF, Watkins LR. Vagal immune-to-brain communication: a visceral chemosensory pathway. Auton Neurosci. 2000;85:49-59.  [PubMed]  [DOI]
3.  Dantzer R, Konsman JP, Bluthé RM, Kelley KW. Neural and humoral pathways of communication from the immune system to the brain: parallel or convergent? Auton Neurosci. 2000;85:60-65.  [PubMed]  [DOI]
4.  Watkins LR, Maier SF, Goehler LE. Cytokine-to-brain communication: a review & amp; analysis of alternative mechanisms. Life Sci. 1995;57:1011-1026.  [PubMed]  [DOI]
5.  Wang X, Wang BR, Ju G. The role of the vagus nerve in transmitting immune information into the brain. Shanghai Mianyixue Zazhi. 2000;20:192-194.  [PubMed]  [DOI]
6.  Lo WF, Ong H, Metcalf ES, Soloski MJ. T cell responses to Gram-negative intracellular bacterial pathogens: a role for CD8+ T cells in immunity to Salmonella infection and the involvement of MHC class Ib molecules. J Immunol. 1999;162:5398-5406.  [PubMed]  [DOI]
7.  Sagar SM, Sharp FR. Early response genes as markers of neuronal activity and growth factor action. Adv Neurol. 1993;59:273-284.  [PubMed]  [DOI]
8.  Xu ZC, Jiang XH. [Recent progress in the use of immediate early genes in neuroscience]. Shengli Kexue Jinzhan. 1997;28:49-51.  [PubMed]  [DOI]
9.  Wang X, Wang BR, Duan XL, Ju G. The basal expression of Fos in the rat under the normal life situation. Zhongguo Shenjing Jiepouxue Zazhi. 2000;16:353-358.  [PubMed]  [DOI]
10.  Matsunaga W, Miyata S, Takamata A, Bun H, Nakashima T, Kiyohara T. LPS-induced Fos expression in oxytocin and vasopressin neurons of the rat hypothalamus. Brain Res. 2000;858:9-18.  [PubMed]  [DOI]
11.  Zhang X, Ju G. [Induction and visualization of c-fos oncogene--a new method reflecting the functional neural pathways]. Shengli Kexue Jinzhan. 1991;22:299-303.  [PubMed]  [DOI]
12.  Arnold FJ, De Lucas Bueno M, Shiers H, Hancock DC, Evan GI, Herbert J. Expression of c-fos in regions of the basal limbic forebrain following intracerebroventricular corticotropin-releasing factor in unstressed or stressed male rats. Neuroscience. 1992;51:377-390.  [PubMed]  [DOI]
13.  Imaki T, Shibasaki T, Hotta M, Demura H. Intracerebroventricular administration of corticotropin-releasing factor induces c-fos mRNA expression in brain regions related to stress responses: comparison with pattern of c-fos mRNA induction after stress. Brain Res. 1993;616:114-125.  [PubMed]  [DOI]
14.  Morgan JI, Cohen DR, Hempstead JL, Curran T. Mapping patterns of c-fos expression in the central nervous system after seizure. Science. 1987;237:192-197.  [PubMed]  [DOI]
15.  Hare AS, Clarke G, Tolchard S. Bacterial lipopolysaccharide-induced changes in FOS protein expression in the rat brain: correlation with thermoregulatory changes and plasma corticosterone. J Neuroendocrinol. 1995;7:791-799.  [PubMed]  [DOI]
16.  Borovikova LV, Ivanova S, Zhang M, Yang H, Botchkina GI, Watkins LR, Wang H, Abumrad N, Eaton JW, Tracey KJ. Vagus nerve stimulation attenuates the systemic inflammatory response to endotoxin. Nature. 2000;405:458-462.  [PubMed]  [DOI]
17.  Ericsson A, Arias C, Sawchenko PE. Evidence for an intramedullary prostaglandin-dependent mechanism in the activation of stress-related neuroendocrine circuitry by intravenous interleukin-1. J Neurosci. 1997;17:7166-7179.  [PubMed]  [DOI]
18.  Ivanov AI, Kulchitsky VA, Sugimoto N, Simons CT, Romanovsky AA. Does the formation of lipopolysaccharide tolerance require intact vagal innervation of the liver? Auton Neurosci. 2000;85:111-118.  [PubMed]  [DOI]
19.  Downing JE, Miyan JA. Neural immunoregulation: emerging roles for nerves in immune homeostasis and disease. Immunol Today. 2000;21:281-289.  [PubMed]  [DOI]
20.  Roth J, De Souza GE. Fever induction pathways: evidence from responses to systemic or local cytokine formation. Braz J Med Biol Res. 2001;34:301-314.  [PubMed]  [DOI]
21.  Watkins LR, Maier SF. Implications of immune-to-brain communication for sickness and pain. Proc Natl Acad Sci USA. 1999;96:7710-7713.  [PubMed]  [DOI]
22.  Shanks N, Windle RJ, Perks PA, Harbuz MS, Jessop DS, Ingram CD, Lightman SL. Early-life exposure to endotoxin alters hypothalamic-pituitary-adrenal function and predisposition to inflammation. Proc Natl Acad Sci USA. 2000;97:5645-5650.  [PubMed]  [DOI]
23.  Ling YL, Meng AH, Zhao XY, Shan BE, Zhang JL, Zhang XP. Effect of cholecystokinin on cytokines during endotoxic shock in rats. World J Gastroenterol. 2001;7:667-671.  [PubMed]  [DOI]
24.  Romanovsky AA. Thermoregulatory manifestations of systemic inflammation: lessons from vagotomy. Auton Neurosci. 2000;85:39-48.  [PubMed]  [DOI]
25.  Blatteis CM, Li S. Pyrogenic signaling via vagal afferents: what stimulates their receptors? Auton Neurosci. 2000;85:66-71.  [PubMed]  [DOI]
26.  Layé S, Bluthé RM, Kent S, Combe C, Médina C, Parnet P, Kelley K, Dantzer R. Subdiaphragmatic vagotomy blocks induction of IL-1 beta mRNA in mice brain in response to peripheral LPS. Am J Physiol. 1995;268:R1327-R1331.  [PubMed]  [DOI]
27.  Hansen MK, Taishi P, Chen Z, Krueger JM. Vagotomy blocks the induction of interleukin-1beta (IL-1beta) mRNA in the brain of rats in response to systemic IL-1beta. J Neurosci. 1998;18:2247-2253.  [PubMed]  [DOI]
28.  Gaykema RP, Dijkstra I, Tilders FJ. Subdiaphragmatic vagotomy suppresses endotoxin-induced activation of hypothalamic corticotropin-releasing hormone neurons and ACTH secretion. Endocrinology. 1995;136:4717-4720.  [PubMed]  [DOI]
29.  Kapcala LP, He JR, Gao Y, Pieper JO, DeTolla LJ. Subdiaphragmatic vagotomy inhibits intra-abdominal interleukin-1 beta stimulation of adrenocorticotropin secretion. Brain Res. 1996;728:247-254.  [PubMed]  [DOI]
30.  Sehic E, Blatteis CM. Blockade of lipopolysaccharide-induced fever by subdiaphragmatic vagotomy in guinea pigs. Brain Res. 1996;726:160-166.  [PubMed]  [DOI]
31.  Gaykema RP, Goehler LE, Tilders FJ, Bol JG, McGorry M, Fleshner M, Maier SF, Watkins LR. Bacterial endotoxin induces fos immunoreactivity in primary afferent neurons of the vagus nerve. Neuroimmunomodulation. 1998;5:234-240.  [PubMed]  [DOI]
32.  Luheshi GN, Bluthé RM, Rushforth D, Mulcahy N, Konsman JP, Goldbach M, Dantzer R. Vagotomy attenuates the behavioural but not the pyrogenic effects of interleukin-1 in rats. Auton Neurosci. 2000;85:127-132.  [PubMed]  [DOI]
33.  Romanovsky AA, Simons CT, Székely M, Kulchitsky VA. The vagus nerve in the thermoregulatory response to systemic inflammation. Am J Physiol. 1997;273:R407-R413.  [PubMed]  [DOI]
34.  Watkins LR, Wiertelak EP, Goehler LE, Smith KP, Martin D, Maier SF. Characterization of cytokine-induced hyperalgesia. Brain Res. 1994;654:15-26.  [PubMed]  [DOI]
35.  Watkins LR, Goehler LE, Relton J, Brewer MT, Maier SF. Mechanisms of tumor necrosis factor-alpha (TNF-alpha) hyperalgesia. Brain Res. 1995;692:244-250.  [PubMed]  [DOI]
36.  Niijima A. The afferent discharges from sensors for interleukin 1 beta in the hepatoportal system in the anesthetized rat. J Auton Nerv Syst. 1996;61:287-291.  [PubMed]  [DOI]
37.  Gordon FJ. Effect of nucleus tractus solitarius lesions on fever produced by interleukin-1beta. Auton Neurosci. 2000;85:102-110.  [PubMed]  [DOI]
38.  Berthoud HR, Neuhuber WL. Functional and chemical anatomy of the afferent vagal system. Auton Neurosci. 2000;85:1-17.  [PubMed]  [DOI]
39.  Dou DB, Cai G. Regulation of the stomach motility function. Shijie Huaren Xiaohua Zazhi. 1999;7:353-354.  [PubMed]  [DOI]
40.  Niedergang F, Sirard JC, Blanc CT, Kraehenbuhl JP. Entry and survival of Salmonella typhimurium in dendritic cells and presentation of recombinant antigens do not require macrophage-specific virulence factors. Proc Natl Acad Sci USA. 2000;97:14650-14655.  [PubMed]  [DOI]
41.  Garcia-del Portillo F, Finlay BB. Salmonella invasion of nonphagocytic cells induces formation of macropinosomes in the host cell. Infect Immun. 1994;62:4641-4645.  [PubMed]  [DOI]
42.  Cookson BT, Bevan MJ. Identification of a natural T cell epitope presented by Salmonella-infected macrophages and recognized by T cells from orally immunized mice. J Immunol. 1997;158:4310-4319.  [PubMed]  [DOI]
43.  Weinstein DL, Carsiotis M, Lissner CR, O'Brien AD. Flagella help Salmonella typhimurium survive within murine macrophages. Infect Immun. 1984;46:819-825.  [PubMed]  [DOI]
44.  Tite JP, Dougan G, Chatfield SN. The involvement of tumor necrosis factor in immunity to Salmonella infection. J Immunol. 1991;147:3161-3164.  [PubMed]  [DOI]
45.  Nauciel C. Role of CD4+ T cells and T-independent mechanisms in acquired resistance to Salmonella typhimurium infection. J Immunol. 1990;145:1265-1269.  [PubMed]  [DOI]
46.  Yang H, Wang L, Ju G. Evidence for hypothalamic paraventricular nucleus as an integrative center of neuroimmunomodulation. Neuroimmunomodulation. 1997;4:120-127.  [PubMed]  [DOI]
47.  Goehler LE, Gaykema RP, Nguyen KT, Lee JE, Tilders FJ, Maier SF, Watkins LR. Interleukin-1beta in immune cells of the abdominal vagus nerve: a link between the immune and nervous systems? J Neurosci. 1999;19:2799-2806.  [PubMed]  [DOI]
48.  Xia B. Pathogeny and mechanism of inflammatory bowel disease. Shijie Huaren Xiaohua Zazhi. 2001;9:245-250.  [PubMed]  [DOI]
49.  Maier SF, Wiertelak EP, Martin D, Watkins LR. Interleukin-1 mediates the behavioral hyperalgesia produced by lithium chloride and endotoxin. Brain Res. 1993;623:321-324.  [PubMed]  [DOI]
50.  Goehler LE, Relton JK, Dripps D, Kiechle R, Tartaglia N, Maier SF, Watkins LR. Vagal paraganglia bind biotinylated interleukin-1 receptor antagonist: a possible mechanism for immune-to-brain communication. Brain Res Bull. 1997;43:357-364.  [PubMed]  [DOI]
51.  Wang X, Wang BR, Duan XL, Liu HL, Ju G. The expression of IL-1receptor type I in nodose ganglion and vagal paraganglion in the rat. Zhongguo Shenjing Kexue Zazhi. 2000;16:90-93.  [PubMed]  [DOI]
52.  Ek M, Kurosawa M, Lundeberg T, Ericsson A. Activation of vagal afferents after intravenous injection of interleukin-1beta: role of endogenous prostaglandins. J Neurosci. 1998;18:9471-9479.  [PubMed]  [DOI]
53.  Wang X, Cao YX, Wang BR, Xu Z, Jin L, Duan XL, Ju G. The influence of subdiaphragmatic vagotomy on CD4 /CD8 T cells in peripheral blood. Xibao Fenzi Yu Mianyixue Zazhi. 2000;16:230-231.  [PubMed]  [DOI]
54.  McSorley SJ, Cookson BT, Jenkins MK. Characterization of CD4+ T cell responses during natural infection with Salmonella typhimurium. J Immunol. 2000;164:986-993.  [PubMed]  [DOI]
55.  Pie S, Truffa-Bachi P, Pla M, Nauciel C. Th1 response in Salmonella typhimurium-infected mice with a high or low rate of bacterial clearance. Infect Immun. 1997;65:4509-4514.  [PubMed]  [DOI]