Transplantation of human hepatocytes into tolerized genetically immunocompetent rats

Figure 6 Quantitation of human albumin DNA in rat livers by dot blotting. Panel A, upper row, shows DNA extracted from liver samples from a intrafetally injected, and transplanted rat at wk6 and 16 post-transplantation, respectively. Lower row shows results from a rat tolerized but without transplantation at the same time points. All dots were hybridized with a ³²P-labeled probe for human albumin DNA. Panel B shows a plasmid, palb-3, containing the complete human albumin gene, applied in decreasing amounts, 100 pg, 10 pg and 1 pg as standards.