APC and K-ras gene mutation in aberrant crypt foci of human colon

Table 1 Primers for K-ras and APC gene sequencing

Genes	Region	Codon	Size of PCR	AT	Sequence
K-ras	exon1	1-54	163	56 °C	5-GACTGAATATAAACTTGTGG
					5-CTGTATCAAAGAAGTGTCCT
K-ras	exon2	31-84	161BP	54 °C	5-GACTGTGTTTCTCCCTTCT
					5-GGCAAATACACAAAGAAAG
APC	15-A	1263-1393	390BP	54 °C	5-GTGTAGAAGATACTCCAATA
					5-GTGAACTGACAGAAGTACAT
APC	15-B	1338-1436	295BP	56 °C	5-CAGGGTTCTAGTTTATCTTC
					5-TTCTGCTTGGTGGCATGGTT
APC	15-C	1412-1515	310BP	56 °C	5-GGAATGGTAAGTGGCATAAT
					5-AAATGGCTCATCGAGGCTCA
APC	15-D	1496-1596	300BP	56 °C	5-ACTCCAGATGGATTTTCTTG
					5-GGCTGGCTTTTTTGCTTTAC