Candida-mediated vertical transmission of Helicobacter pylori in C57BL/6J mice

Table 1 Primers used to detect Helicobacter pylori and Candida strains

Gene	Sequence	Polymerase chain reaction product size (bp)	Procedure	Ref.
16S rDNA	HeliS: AAGAACCTTACCTAGGCTTGACATTG	497	94 °C 3 minutes; 37 cycles at 94 °C 45 seconds, 57 °C 60 seconds, 72 °C 60 seconds; 72 °C 5 minutes	[35]
	HeliN: GCTAAGAGATCAGCCTATGTCC
	Hpup: TGAGAGAATCCGCTAGAAATAGTGG	454	94 °C 3 minutes; 25 cycles at 94 °C 45 seconds, 57 °C 1 minutes, 72 °C 1 minutes; 72 °C 5 minutes
	Hpdown: TAGCATCCTGACTTAAGGCAAACA
ureA	PylF: CCAGATGATGTGATGGATGG	607	94 °C 3 minutes; 25 cycles at 94 °C 45 seconds; 50 °C 45 seconds, 72 °C 3 minutes; 72 °C 5 minutes	[36]
	PylR: TCAAGTCTGTATCGCCCAATC
	HpU1: GCCAATGGTAAATTAGTT	411	94 °C 3 minutes; 34 cycles at 94 °C 45 seconds, 45 °C 45 seconds, 72 °C 45 seconds; 72 °C 5 minutes
	HpU2: CTCCTTAATTGTTTTTAC
ITS	ITS1: TCCGTAGGTGAACCTGCGG	500-800	94 °C 5 minutes; 35 cycles at 94 °C 1 minutes; 60 °C 30 seconds; 72 °C 1 minutes; 72 °C 10 minutes	[37]
	ITS4: TCCTCCGCTTATTGATATGC

ITS: Internal transcribed spacer; ureA: Urease A.