Specificity protein 1/ZIP8/zinc axis reduced macrophage extracellular traps to alleviate severe acute pancreatitis in mice

Figure 6 The overexpression of ZIP8 mitigates the severity of severe acute pancreatitis by decreasing macrophage extracellular trap formation. A: Schematic diagram of the experimental procedure; B: Western blot analysis of ZIP8 protein expression levels in peritoneal macrophages (n = 4); C: Quantitative real-time polymerase chain reaction analysis of ZIP8 messenger RNA expression levels in peritoneal macrophages (n = 7); D: Representative immunofluorescence images showing ZIP8 (green) and cluster of differentiation 68 (red) expression; E and F: Hematoxylin-eosin staining demonstrating the pathological effects of ZIP8 overexpression on pancreatic and lung tissues; G and H: Plasma levels of amylase and lipase (n = 7); I: Plasma levels of double-stranded DNA (n = 7); J: Representative fluorescence images of citrullinated histone H3 (CitH3) and F4/80 in the pancreas, together with quantitative analysis of CitH3 fluorescence intensity (n = 7). ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. AAV: Adeno-associated virus; Ctrl: Control; DAPI: 4’,6-diamidino-2-phenylindole; mRNA: Messenger RNA; CD: Cluster of differentiation; CitH3: Citrullinated histone H3; dsDNA: Double-stranded DNA; MFI: Mean fluorescence intensity.