Specificity protein 1/ZIP8/zinc axis reduced macrophage extracellular traps to alleviate severe acute pancreatitis in mice

Figure 2 Degradation of macrophage extracellular traps alleviates the severity of severe acute pancreatitis. A: Experimental flowchart: C57 mice were pretreated with anti-Ly6G antibody, followed by induction of severe acute pancreatitis and treatment with DNase I; B: Representative hematoxylin-eosin-stained images of pancreatic and lung tissues; C and D: Expression levels of amylase and lipase in plasma (n = 7); E and F: Representative immunohistochemical images of cleaved caspase 3 (brown granules) in pancreatic tissue, accompanied by statistical analysis (n = 7); G: Expression level of 16S rDNA in plasma (n = 4); H: Plasma fluorescein isothiocyanate (FITC) signal in each group of mice following oral gavage with FITC-dextran (n = 4). ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. NS: Not significant; Ctrl: Control; DNase I: Deoxyribonuclease I; Casp: Caspase; IHC: Immunohistochemical; 16S rDNA: 16S ribosomal DNA; FITC: Fluorescein isothiocyanate.