Specificity protein 1/ZIP8/zinc axis reduced macrophage extracellular traps to alleviate severe acute pancreatitis in mice

Figure 1 Macrophage extracellular traps are present in severe acute pancreatitis. A: Hematoxylin-eosin staining revealed pathological changes in the pancreatic and lung tissues in acute pancreatitis models induced by caerulein, and in severe acute pancreatitis (SAP) models induced by either caerulein or duct ligation; B: Levels of plasma amylase and lipase (n = 4); C: Immunofluorescence staining revealed the protein expression levels of citrullinated histone H3 (CitH3) (red fluorescence) and F4/80 (green fluorescence) in SAP; D: Levels of double-stranded DNA (dsDNA) in plasma (n = 4); E and F: Neutrophils were depleted from mice using an anti-Ly6G antibody, followed by induction of the SAP model. Cluster of differentiation (CD) 11b and Ly6G antibodies were subsequently used for staining, and flow cytometry was performed to determine the purity of CD11b and Ly6G in blood and pancreatic tissues; G: Levels of dsDNA in plasma (n = 5); H: After pretreatment with an anti-Ly6G antibody, the expression of F4/80 and CitH3 in pancreatic tissue was assessed by immunofluorescence. ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. NS: Not significant; Nc: Negative control; AP: Acute pancreatitis; SAP: Severe acute pancreatitis; CitH3: Citrullinated histone H3; DAPI: 4’,6-diamidino-2-phenylindole; dsDNA: Double-stranded DNA.