Wenyang Jiedu Huayu Formula targets succinate metabolism to alleviate acute-on-chronic liver failure

Figure 6 Wenyang Jiedu Huayu Formula inhibits macrophage infiltration in the liver of acute-on-chronic liver failure rats. A: Representative immunofluorescence images of liver sections from the normal control, Model, Wenyang Jiedu Huayu Formula, and WYIN (Wenyang Jiedu Huayu Formula plus dimethyl malonate) groups stained for CD68 (green) and Ki67 (red). Nuclei were counterstained with DAPI (blue). Scale bar: 20 μm; B: Quantitative analysis of CD68+ area percentage and Ki67 labeling index. CD68+ area was calculated as the percentage of CD68-positive area relative to the total tissue area. The Ki67 labeling index was calculated as (Ki67 nuclei/DAPI total nuclei) × 100%. Data are presented as mean ± SD. ^bP < 0.01, ^dP < 0.0001. NC: Normal control group; WYJD: Wenyang Jiedu Huayu Formula; WYIN: Wenyang Jiedu Huayu Formula plus dimethyl malonate.