OH-CATH30 targets CD40 to suppress NF-κB signaling and promote arachidonic acid metabolic reprogramming in acute pancreatitis

Figure 3 Transcriptomic profiling shows that OH-CATH30 modulates inflammatory signaling pathways in acute pancreatitis. A and B: Volcano plots depicting differentially expressed genes in pancreatic tissues from (A) acute pancreatitis (AP) vs control and (B) OH-CATH30 vs AP comparisons. Red and blue dots represent significantly upregulated and downregulated genes, respectively (adjusted P ≤ 0.05, |log₂(fold change)| ≥ 1). Gray dots indicate non-significant changes; C and D: Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of differentially expressed genes; C: Top enriched pathways in AP vs control; D: Pathways significantly modulated by OH-CATH30 treatment; E: Heatmap showing relative abundance of infiltrating immune cell types in each sample, estimated using CIBERSORT algorithm; F: Bar plot depicting average immune cell composition in control, AP, and OH-CATH30-treated groups. All data are derived from transcriptomic sequencing of pancreatic tissues (n = 4 biological replicates per group). AP: Acute pancreatitis; KEGG: Kyoto Encyclopedia of Genes and Genomes.