OH-CATH30 targets CD40 to suppress NF-κB signaling and promote arachidonic acid metabolic reprogramming in acute pancreatitis

Figure 2 OH-CATH30 attenuates cerulein-induced acute pancreatitis in mice. A-D: Time-course analysis of serum pro-inflammatory cytokines in cerulein-induced acute pancreatitis (AP) mice. Mice were euthanized at 0, 12, 24, 48, 72, and 96 hours after the last cerulein injection (n = 3 per time point). Serum levels of interleukin (IL)-1β (A), IL-6 (B), IL-18 (C), and tumor necrosis factor-α (D) were measured using ELISA. ^aP < 0.05 vs 0 hour, ^bP < 0.05 vs 12 hours, ^cP < 0.05 vs 24 hours, ^dP < 0.05 vs 48 hours, and ^eP < 0.05 vs 72 hours; E-M: Therapeutic effects of OH-CATH30 on AP mice. Mice were randomly assigned to three groups: Control (phosphate-buffered saline only), AP (10 hourly cerulein injections), and AP + OH-CATH30 (30 μg/kg i.p. every 12 hours for 3 days, starting immediately after sixth cerulein injection). Pancreatic tissues and serum were collected 24 hours after final cerulein injection. ^aP < 0.05 vs control, ^bP < 0.05 vs acute pancreatitis group. Serum levels of IL-1β, IL-6, IL-18, and tumor necrosis factor-α measured using ELISA (E-H); serum amylase and lipase activities (I and J); lactate dehydrogenase activity in pancreatic tissue homogenates (K); representative hematoxylin and eosin-stained pancreatic sections (L) and corresponding histological scores (M). Scale bar = 100 μm. All data are presented as mean ± SD (n = 6 mice/group). Statistical analysis was performed using one-way ANOVA followed by Tukey’s HSD post hoc test. IL: Interleukin; TNF-α: Tumor necrosis factor-α; AP: Acute pancreatitis; LDH: Lactate dehydrogenase.