Depleting apolipoprotein C3 alleviates acute pancreatitis in hamsters but not in mice

Figure 3 Apolipoprotein C3 deficiency alleviates ethanol + palmitoleic acid-induced acute pancreatitis in hamsters. A: Schematic of the experimental protocol: 8- to 9-week-old male hamsters were intraperitoneally injected with ethanol (EtOH) (1.35 g/kg) and palmitoleic acid (POA) (150 mg/kg) to induce pancreatitis. Blood samples were collected at 0, 6, 9, 12, and 24 hours, and the animals were sacrificed at 24 hours; B: Plasma triglyceride levels were measured across treatment groups, n = 3-5; C: Plasma amylase activity was assessed at multiple time points in wild type and apolipoprotein C3 knockout hamsters with or without EtOH + POA, n = 3-5; D: Representative hematoxylin and eosin-stained pancreatic tissue sections, with enlarged views shown below; E: Quantitative histological scoring of pancreatic tissue, evaluating oedema, inflammation, necrosis, and total pathology score, n = 3-5. Data are presented as the mean ± SEM. ^aP < 0.05, ^bP < 0.01 and ^cP < 0.001. ApoC3: Apolipoprotein C3; ApoC3^-/-: Apolipoprotein C3 knockout; AP: Acute pancreatitis; EtOH: Ethanol; POA: Palmitoleic acid; HE: Hematoxylin and eosin.