Exosomes derived from human umbilical cord mesenchymal stem cells attenuate hepatic ischaemia-reperfusion injury via the let-7i-5p/Faslg axis

Figure 5 Let-7i-5p in human umbilical cord mesenchymal stem cell-derived exosomes affects the level of LO2 cell apoptosis after hypoxia/reoxygenation injury by regulating the expression of factor-related apoptosis ligand. A: Representative light microscopy images of LO2 cells after hypoxia/reoxygenation (H/R) treatment in the cell rescue experiment. Scale bars: 100 μm; B: Flow cytometry analysis of the apoptosis rate of LO2 cells after H/R treatment in the cell rescue experiment; C: Western blot analysis of the protein levels of apoptosis-related markers in LO2 cells after H/R treatment in the cell rescue experiment. H/R: Hypoxia/reoxygenation; hucMSC-exos: Human umbilical cord mesenchymal stem cell-derived exosomes; Faslg-oe: Lentiviral vectors overexpressing factor-related apoptosis ligand; NC: Negative control; Bcl-2: B-cell lymphoma-2; Bax: B-cell lymphoma-2-associated X protein; Caspase-3: Cysteinyl aspartate specific proteinase-3; Faslg: Factor-related apoptosis ligand. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, and ^dP < 0.0001.