Exosomes derived from human umbilical cord mesenchymal stem cells attenuate hepatic ischaemia-reperfusion injury via the let-7i-5p/Faslg axis

Figure 4 Role of factor-related apoptosis ligand-mediated let-7i-5p in human umbilical cord mesenchymal stem cell-derived exosomes in regulating the apoptosis of LO2 cells after hypoxia/reoxygenation injury. A: Representative light microscopy images of LO2 cells infected with lentiviral vectors after hypoxia/reoxygenation (H/R) treatment. Scale bars: 100 μm; B: Flow cytometry was used to detect the apoptosis rate of LO2 cells after lentiviral vector infection followed by H/R treatment; C: Western blot analysis was performed to detect the protein levels of apoptosis-related markers in LO2 cells after factor-related apoptosis ligand overexpression or factor-related apoptosis ligand knockdown followed by H/R treatment. H/R: Hypoxia/reoxygenation; Faslg-oe: Lentiviral vector overexpressing factor-related apoptosis ligand; Faslg-sh: Factor-related apoptosis ligand short hairpin RNA, lentiviral RNA interference vector-mediated factor-related apoptosis ligand; NC: Negative control; Bcl-2: B-cell lymphoma-2; Bax: B-cell lymphoma-2-associated X protein; Caspase-3: Cysteinyl aspartate specific proteinase-3; Faslg: Factor-related apoptosis ligand; 7-AAD: 7-aminoactinomycin D. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, and ^dP < 0.0001.