Exosomes derived from human umbilical cord mesenchymal stem cells attenuate hepatic ischaemia-reperfusion injury via the let-7i-5p/Faslg axis

Figure 3 Role of let-7i-5p in the effects of human umbilical cord mesenchymal stem cell-derived exosomes on the apoptosis of LO2 cells after hypoxia/reoxygenation injury. A: Representative fluorescence images showing the uptake of transfected human umbilical cord mesenchymal stem cell-derived exosomes (hucMSC-exos) by LO2 cells after hypoxia/reoxygenation (H/R) injury. Scale bars: 25 μm; B: After the coincubation of H/R-treated LO2 cells with transfected hucMSC-exos, quantitative real-time polymerase chain reaction was used to detect the expression levels of let-7i-5p in LO2 cells; C: Representative light microscopy images of H/R-treated LO2 cells coincubated with hucMSC-exos overexpressing or inhibiting let-7i-5p. Scale bars: 100 μm; D: Flow cytometry was used to detect the apoptosis rate of LO2 cells after the coincubation of transfected hucMSC-exos with H/R-treated LO2 cells; E: Western blot analysis of changes in the expression levels of apoptosis-related protein markers in LO2 cells after the coincubation of transfected hucMSC-exos with H/R-treated LO2 cells. H/R: Hypoxia/reoxygenation; DAPI: 4’,6-diamidino-2-phenylindole; Cy3: Cyanine3; NC: Negative control; Bax: B-cell lymphoma-2-associated X protein; Bcl-2: B-cell lymphoma-2; Caspase-3: Cysteinyl aspartate specific proteinase-3; Faslg: Factor-related apoptosis ligand; 7-AAD: 7-aminoactinomycin D. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, and ^dP < 0.0001.