TSC22D1 promotes liver sinusoidal endothelial cell dysfunction and induces macrophage M1 polarization in non-alcoholic fatty liver disease

Figure 7 Targeting TSC22D1 alleviates liver sinusoidal endothelial cells dysfunction and inhibits macrophage M1 polarization via the tumor necrosis factor-like weak inducer of apoptosis/fibroblast growth factor-inducible 14 pathway in vivo. A: The interference efficiency of different short hairpin RNAs against TSC22D1 via quantitative real-time polymerase chain reaction analysis; B: Hematoxylin eosin, Sirius red, and Oil red O staining of liver tissues from the adeno-associated virus serotype 8-short hairpin RNA-negative control group and the recombinant adeno-associated virus serotype 8 carrying TSC22D1-targeting short hairpin RNA vector group; C: Fluorescence double staining for cluster of differentiation (CD) 31 and TSC22D1; D: Immunohistochemical staining for CD34, laminin, angiotensin-2, α-smooth muscle actin, E-cadherin, and N-cadherin in liver tissues; E: Immunohistochemical staining for mouse epidermal growth factor-like module-containing mucin-like hormone receptor-like 1, inducible nitric oxide synthase, and CD206; F: Western blot analysis for TSC22D1 and tumor necrosis factor-like weak inducer of apoptosis/fibroblast growth factor-inducible 14 pathway. Scale bar 100 μm, 100 × magnification. Scale bar 50 μm, 200 × magnification. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001. Student’s t test. n = 6 mice each group. sh-TSC22D1: The recombinant adeno-associated virus serotype 8 carrying TSC22D1-targeting short hairpin RNA vector; sh-NC: The recombinant adeno-associated virus serotype 8 carrying negative control short hairpin RNA vector; F4/80: Mouse epidermal growth factor-like module-containing mucin-like hormone receptor-like 1; HE: Hematoxylin eosin; CD: Cluster of differentiation; DAPI: 4’,6-diamidino-2-phenylindole; Ang-2: Angiotensin-2; SMA: Smooth muscle actin; iNOS: Inducible nitric oxide synthase; NS: No significant; TWEAK: Tumor necrosis factor-like weak inducer of apoptosis; FN14: Fibroblast growth factor-inducible 14; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.