Tetrahydroxylated bile acids prevents malignant progression of Barret esophagus in vitro by inhibiting the interleukin-1β-nuclear factor kappa-B pathway

Figure 3 Effects of deoxycholic acid and tetrahydroxylated bile acids co-exposure on adult tissue-resident stem cells isolated from Barrett esophagus clinical biopsies mucin production. Representative bright field images of Barrett esophagus clinical biopsies (BE-ASCs) in transwell inserts. A: BE-ASCs treated with 2000 μM tetrahydroxylated bile acids restored mucin production (the level of secretion was 17-fold higher in comparison to the cells treated with 150 μM deoxycholic acid alone); B: Mucin levels were correlated with dark density measured by ImageJ software. Data are presented as mean ± SD (n = 3). ^dP < 0.0001. DCA: Deoxycholic acid; THBA: Tetrahydroxylated bile acids.