Translocator protein facilitates neutrophil-mediated mucosal inflammation in inflammatory bowel diseases

Figure 1 Bioinformatics analysis of translocator protein expression in inflammatory bowel diseases. A: Gene Expression Omnibus (GEO) dataset GSE75214 uses gene microarray technology to analyze gene expression profiles of endoscopic biopsy specimens from patients with inflammatory bowel diseases. For Crohn's disease (CD), ileal mucosal data comprising 11 healthy controls (HC), 16 remission stage, and 51 active stage specimens, were used, while for ulcerative colitis (UC), colon mucosal data were analyzed, including 11 HC, 23 remission stage, and 74 active stage specimens; B-D: GEO dataset GSE94648 includes 22 HC, 25 patients with UC, and 50 patients with CD, the gene expression profile of peripheral whole blood is analyzed using gene microarray technology; E and F: The single-cell sequencing dataset (GSE156776) includes crawling adipose tissue samples from 2 patients with CD and mesenteric adipose tissue samples from 2 HC. The Seurat package was used to perform dimensionality reduction and clustering on the original data, and the subpopulations corresponding to immune cells (including T cells, natural killer cells, B cells, plasma cells, neutrophils, macrophages, and dendritic cells) are then extracted and analyzed; the expression of TSPO is compared in CD and HC neutrophils. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. CD: Crohn's disease; DC: Dendritic cells; HC: Healthy controls; NK: Natural killer; TSPO: Translocator protein; UC: Ulcerative colitis.