Myeloperoxidase, extracellular DNA and neutrophil extracellular trap formation in the animal models of metabolic dysfunction-associated steatotic liver disease

Figure 6 Thioacetamide-induced model blood cell count and neutrophil extracellular trap-associated peripheral markers. A: White blood cell count; B: Lymphocyte count; C: Neutrophil count; D: Concentration of myeloperoxidase; E: Concentration of neutrophil elastase; F: Myeloperoxidase-DNA complex count; G: NETotic neutrophil count; H: Concentration of extracellular DNA (ecDNA); I: DNase activity; J: Concentration of nuclear DNA; K: Concentration of mitochondrial DNA; L: Concentration of small (20-250 bp) fragments of circulating ecDNA; M: Concentration of large (250-1000 bp) fragments of circulating ecDNA; N: Ratio between small and large fragments of circulating ecDNA after 10 weeks. Data are presented as mean ± SE (A-M) or standard deviation (N). ^aP < 0.05; ^bP < 0.01; ^cP < 0.001; A-M: Repeated two-way analysis of variance and Bonferroni post-hoc test; N: Independent Student’s t-test. VEH: Control group receiving intraperitoneal saline injections (n = 7); TAA: Experimental liver fibrosis group receiving intraperitoneal thioacetamide injections (n = 11). WBC: White blood cell; LYM: Lymphocytes; NEU: Neutrophils; MPO: Myeloperoxidase; NE: Neutrophil elastase; NET: Neutrophil extracellular trap; ecDNA: Extracellular DNA; ncDNA: Nuclear DNA; mtDNA: Mitochondrial DNA; TAA: Thioacetamide; CTRL: Control group.