Kushenol I combats ulcerative colitis via intestinal barrier preservation and gut microbiota optimization

doi:10.3748/wjg.v31.i26.105656

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World Journal of Gastroenterology

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1007-9327

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World J Gastroenterol. Jul 14, 2025; 31(26): 105656
Published online Jul 14, 2025. doi: 10.3748/wjg.v31.i26.105656

Table 2 Primer sequences used for the amplification of each target gene using polymerase chain reaction, along with the corresponding lengths of the amplified fragments

Target gene	Amplified fragment length/bp	Primer sequences
IL-17	75	Upstream: 5’-TGATGCTGTTGCTGCTGCTGAG-3’
IL-17	75	Downstream: 5’-GACACGCTGAGCTTTGAGGGATG-3’
IL-10	81	Upstream: 5’-GGACAACATACTGCTAACCGACTC-3’
IL-10	81	Downstream: 5’-TGGATCATTTCCGATAAGGCTTGG-3’
TNF-α	118	Upstream: 5’-CCACCACGCTCTTCTGTCTACTG-3’
TNF-α	118	Downstream: 5’-TGGTTTGTGAGTGTGAGGGTCTG-3’
IL-1β	145	Upstream: 5’-CACTACAGGCTCCGAGATGAACAAC-3’
IL-1β	145	Downstream: 5’-TGTCGTTGCTTGGTTCTCCTTGTAC-3’
IL-6	91	Upstream: 5’-CTTCTTGGGACTGATGCTGGTGAC-3’
IL-6	91	Downstream: 5’-TCTGTTGGGAGTGGTATCCTCTGTG-3’

IL: Interleukin; TNF: Tumor necrosis factor.

Citation: He XD, Li M, Zuo XD, Ni HY, Han YX, Hu YK, Yu J, Yang XX. Kushenol I combats ulcerative colitis via intestinal barrier preservation and gut microbiota optimization. World J Gastroenterol 2025; 31(26): 105656
URL: https://www.wjgnet.com/1007-9327/full/v31/i26/105656.htm
DOI: https://dx.doi.org/10.3748/wjg.v31.i26.105656