Perisurgical colony stimulating factor one treatment ameliorates liver ischaemia/reperfusion injury in rats

Figure 3 Colony stimulating factor 1-Fc treatment reduced the extent of histological necrosis, augmented liver macrophage numbers and promoted cell proliferation. A and B: Suzuki Score (A) and necrotic area (B) assessed by image analysis in ischaemic lobes 48 hours post reperfusion; C: Caspase 3 activity in liver lysates, assessed cleavage of a fluorescent substrate. Liver lysates from age-matched Dark Agouti rats (n = 3) that had not been subjected to ischemia-reperfusion surgery collected at a different time were used as untreated controls. Kruskal Wallis test with Dunn’s multiple comparisons test (not significant); D and E: Representative immunohistochemistry images of liver IBA1+ cells (D) and quantification of IBA1+ intranecrotic staining area (E), zonation band thickness and IBA1+ staining area in the ischemic and oxygenated lobe (OL). The zonation band was not quantified in 3 rats -1 saline, 2 colony stimulating factor (CSF)1-Fc in which no distinct necrotic area was present; F and G: Representative immunohistochemistry images of liver Ki67 staining (F) and quantification of Ki67 positive hepatocytes and other cells in the ischemic lobe (IL) and OL (G); H: Representative immunohistochemistry images showing Ki67+ cells at a higher magnification in the peri-necrotic zone in the ischemic lobes for saline and CSF1-Fc treatment. Data show mean and standard deviation. Data were analysed using Mann Whitney test, except for (C). ^aP < 0.05, ^bP < 0.01. FI: Fluorescent intensity; IL: Ischemic lobe; OL: Oxygenated lobe; CSF: Colony stimulating factor.