Perisurgical colony stimulating factor one treatment ameliorates liver ischaemia/reperfusion injury in rats

Figure 1 Partial liver ischemia-reperfusion injury induced endogenous colony stimulating factor 1 24 hours post reperfusion. Groups of 4–6-week-old rats (n = 5/group, mixed gender, females indicated by triangle symbol) were subjected to 70% hepatic ischemia for 45 minutes and euthanized at 6-, 24-, 48- and 72-hours post reperfusion. A: Representative H&E sections of formalin-fixed paraffin-embedded livers showing different stages of ischemic injury at 6-72 hours; B: Macroscopic lesion on the left lateral and median liver lobes at 48 hours post ischemia-reperfusion; C-E: Suzuki Scores (C), serum aspartate aminotransferase and alanine aminotransferase (D), serum bilirubin, gamma glutamyl transferase and alkaline phosphatase (E). Grey line indicates limit of detection; F: Endogenous serum CSF1 was measured by ELISA; G: Monocyte count in the peripheral blood was quantified using a haematology analyser. Data show mean and standard deviation. Results were analysed with the Kruskal Wallis test; ^aP < 0.05, ^bP < 0.01. ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; CSF: Colony stimulating factor.