miR-10a-5p and miR-10b-5p restore colonic motility in aged mice

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Jun 28, 2025 (publication date) through Jun 26, 2025

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Basic Study

World J Gastroenterol. Jun 28, 2025; 31(24): 104437
Published online Jun 28, 2025. doi: 10.3748/wjg.v31.i24.104437

Table 1 Oligonucleotides used in this study: Primers

Name	Sequences (5' to 3')	Sense	Product (bp)	Spices	Application
miRTQ	CGAATTCTAGAGCTCGAGGCAGGCGACATGGCTGGCTAGTTAAGCTTGGTACCGAGCTCGGATCCACTAGTCCTTTTTTTTTTTTTTTTTTTTTTTTTVN¹	Reverse			cDNA
miRTQ-rev	CGAATTCTAGAGCTCGAGGCAGG	Reverse			qPCR
miR-10a	TACCCTGTAGATCCGAATTTG	Forward	123	Mouse/human	qPCR
miR-10b	TACCCTGTAGAACCGAATTTG	Forward	123	Mouse/human	qPCR
Snord55	GGTAATGCTGCATACTCCCGAG	Forward		Human	qPCR
Snord66	CTGAGACCACATGATGGGATTG	Forward		Mouse	qPCR
M13	CAGGAAACAGCTATGAC	Forward	289		Cloning
M13-rev	ACTGGCCGTCGTTTTAC	Reverse	289		Cloning

¹In RTQ primer, V is A, G, or C; N is A, G, C, or T.

Citation: Baek G, Singh R, Ha SE, Cho H, Padmanabhan S, Vishwanath V, Kim MS, Seon D, You J, Lee MY, Ro S. miR-10a-5p and miR-10b-5p restore colonic motility in aged mice. World J Gastroenterol 2025; 31(24): 104437
URL: https://www.wjgnet.com/1007-9327/full/v31/i24/104437.htm
DOI: https://dx.doi.org/10.3748/wjg.v31.i24.104437