Endothelin A receptor in nociceptors is essential for persistent mechanical pain in a chronic pancreatitis of mouse model

Figure 5 A higher sensitivity in primary sensory neurons and histological characterization of mice with chronic pancreatitis. A and B: Less sensitive to endothelin-1 in dissociated T8-12 dorsal root ganglion neurons from conditional knockout (CKO) mice with chronic pancreatitis than those from wild type (WT) mice with chronic pancreatitis measured by calcium imaging for the degree of calcium influx into the neurons (A) and the percentage of responsive cells from five mice (B). n = 36-69 neurons from 5 mice/group; C-E: Representative hematoxylin and eosin staining images of mouse pancreas sections from WT-vehicle group (C), WT-dibutyltin dichloride group (D), and CKO-dibutyltin dichloride group (E); F: The percentage of body weight change for experimental animals around 4 weeks. Scale bar, 50 μm (A) and 200 μm (C-E). n = 10 mice. Data are shown as mean ± SEM. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, statistical comparison were conducted with one-way analysis of variance with Sidak’s post hoc test (A and F) or χ²-test (B). WT: Wild type; CKO: Conditional knockout; D: Dibutyltin dichloride (DBTC); ET-1: endothelin-1; NS: Not significant; V: Vehicle.