Endothelin A receptor in nociceptors is essential for persistent mechanical pain in a chronic pancreatitis of mouse model

Figure 4 Increased macrophage infiltration into the DRG and microglial activation in the spinal cord of mice with chronic pancreatitis. A and B: Representative images of immunofluorescence staining of ionized calcium-binding adapter molecule 1 (IBA-1) on the T8-12 dorsal root ganglion neurons (A) and spinal cord (B); C: The number of IBA-1-positive cells; D: Relative IBA-1-positive density (normalized to WT-vehicle group) in the dorsal spinal cord was analyzed for each group of mice. Scale bar, 50 μm (A) and 100 μm (B). n = 5 mice. Data are shown as mean ± SEM. ^bP < 0.01, ^cP < 0.001, statistical comparison were conducted with one-way analysis of variance with Sidak’s post hoc test. WT: Wild type; CKO: Conditional knockout; DBTC: Dibutyltin dichloride; DRG: Dorsal root ganglion; IBA-1: Ionized calcium binding adaptor molecule 1.