Endothelin A receptor in nociceptors is essential for persistent mechanical pain in a chronic pancreatitis of mouse model

Figure 2 Behavioral phenotypes of dibutyltin dichloride -induced chronic pancreatitis model. A: The response thresholds to abdominal mechanical stimulation were measured in experimental mice before and after chronic pancreatitis induction with von Frey filaments; B: Von Frey filament with 0.4-g force was applied to mouse abdomen and the response frequencies of mice were calculated; C: The abdominal response latencies to radiant heat were determined in mice from different groups; D: Acetone-induced nociceptive response events were counted to reflect mouse abdominal responses to cold stimuli; E: The fall latencies of mice were determined to reflect mouse motor abilities in rotarod test; F and G: The total travelled distance of mice was measured to reflect mouse motor activities (F) and the duration the central zone was recorded to reflect mouse mood alteration (G) in the open field test; H and I: The percentages of open arm entries (H) and duration in the open arm (I) were measured to reflect mouse anxiety-like behaviors in the EPM test. n = 10 mice. Data are shown as mean ± SEM. ^bP < 0.01, ^cP < 0.001, statistical comparisons were conducted with unpaired two-way analysis of variance (A-D) or one-way analysis of variance (E-I) with Sidak’s post hoc test. WT: Wild type; CKO: Conditional knockout; DBTC: Dibutyltin dichloride; EPM: Elevated plus maze.