Intermittent fasting exacerbates colon inflammation by promoting Th17 cell differentiation through inhibition of gut microbiota-derived indoleacrylic acid

Figure 4 Combined analysis of non-targeted metabolomics and 16s rRNA sequencing. Mouse intestinal contents by DNA extraction, polymerase chain reaction amplification, sequencing analysis, flora composition and diversity, at the same time by the metabolite spectrum and pathway analysis, then the two content joint analysis, cluster analysis, shows a significant difference between the two groups, finally the interaction network, indicating that the core flora (e.g. Alistipes and Unidentified _ g _ UCG-010) and a variety of metabolites established a strong correlation. NC: Normal control; IF: Intermittent fasting; PCR: Polymerase chain reaction.