Intermittent fasting exacerbates colon inflammation by promoting Th17 cell differentiation through inhibition of gut microbiota-derived indoleacrylic acid

Figure 3 Restrictive diet is associated with gut microbiota dysbiosis. A: The abundance-based coverage estimator and Chao1 index analysis, found significant differences in α diversity between the two groups; B: Principal component analysis showed significant differences in β diversity observed in the two groups; C: A total number of 369 species in both groups of gut microbiota, of the 375 species endemic to the normal control group, 292 species; D: At the gate level, Firmicutes (Firmicutes) decreased and Cyclospora (Campilobacterota) increased in the intermittent fasting group; At the genus level, the relative abundance of group F species (Rikenella) and murobacteria (Muribaculaceae) increased; E and F: Lefse further analyzed the microbial communities. ^bP < 0.01. NC: Normal control; IF: Intermittent fasting; PC: Principal component; LDA: Linear discriminant analysis.