N6-methyladenosine reader IGF2BP2 regulates NIPSNAP1-mediated mitophagy and mitochondrial dynamics to alleviate hepatic ischemia-reperfusion injury

Figure 5 The protective effect of interacted with insulin-like growing factor 2 overexpression in hepatic ischemia-reperfusion is suppressed by 4-nitrophenylphosphatase domain and non-neuronal SNAP25-like protein homolog 1 knockdown. ^aP < 0.05, ^bP < 0.01. A: Hematoxylin eosin staining, n = 6. Scale bar: 100 μm; B: Alanine aminotransferase and aspartate aminotransferase activities, n = 6; C: Mitochondrial morphology was assessed using transmission electron microscopy (× 40000, magnification); D: Adeno-associated virus 9-mediated delivery was used to generate 4-nitrophenylphosphatase domain and non-neuronal SNAP25-like protein homolog 1 (NIPSNAP1) shRNA - and interacted with insulin-like growing factor 2-overexpressing mice, which were subsequently subjected to ischemia-reperfusion. NIPSNAP1, MAP1LC3 II/I, p62, mitofusin 2, mitochondrial fission 1 protein, and phosphorylated dynamin-related protein 1/dynamin-related protein 1 (Drp1) proteins, n = 3; E: HepG2 cells were subjected to hypoxia/reoxygenation after cotransfection with pcDNA-interacted with insulin-like growing factor 2 and NIPSNAP1 small interfering RNAs. NIPSNAP1, MAP1LC3 II/I, p62, mitofusin 2, mitochondrial fission 1 protein, and phosphorylated-Drp1/Drp1 proteins, n = 3. HE: Hematoxylin eosin; NIPSNAP1: 4-nitrophenylphosphatase domain and non-neuronal SNAP25-like protein homolog 1; AAV: Adeno-associated virus; I/R: Ischemia-reperfusion; IGF2BP2: Insulin-like growth factor 2 mRNA-binding protein 2; TBG: Thyroxine-binding globulin; TEM: Transmission electron microscopy; MFN2: Mitofusin 2; FIS1: Mitochondrial fission 1 protein; Drp1: Dynamin-related protein 1; p-Drp1: Phosphorylated dynamin-related protein 1; LC3: MAP1LC3; NC: Negative control.