N6-methyladenosine reader IGF2BP2 regulates NIPSNAP1-mediated mitophagy and mitochondrial dynamics to alleviate hepatic ischemia-reperfusion injury

Figure 3 4-nitrophenylphosphatase domain and non-neuronal SNAP25-like protein homolog 1 regulates mitochondrial dynamics in hepatic ischemia-reperfusion. ^aP < 0.05, ^bP < 0.01. A: Mitochondrial morphology was assessed using transmission electron microscopy (× 40000, magnification); B: Co-immunoprecipitation was used to verify the interaction between 4-nitrophenylphosphatase domain and non-neuronal SNAP25-like protein homolog 1 (NIPSNAP1) and MFN2 or FIS1 in the livers of mice; C: Liver MFN2, FIS1, dynamin-related protein 1, and phosphorylated dynamin-related protein 1 (s616) proteins, n = 3; D and E: HepG2 cells were exposed to hypoxia/reoxygenation after transfection with NIPSNAP1 small interfering RNAs or pcDNA-NIPSNAP1. MFN2, FIS1, Drp1 and phosphorylated dynamin-related protein 1(s616) proteins, n = 3. AAV: Adeno-associated virus; I/R: Ischemia-reperfusion; NIPSNAP1: 4-nitrophenylphosphatase domain and non-neuronal SNAP25-like protein homolog 1; TEM: Transmission electron microscopy; NC: Negative control; IP: Immunoprecipitation; MFN2: Mitofusin 2; FIS1: Mitochondrial fission 1 protein; Drp1: Dynamin-related protein 1; p-Drp1: Phosphorylated dynamin-related protein 1; H/R: Hypoxia/reoxygenation.