Multi-parameter magnetic resonance imaging of zebularine in liver fibrosis treatment and calcineurin/NFAT3 mechanism

doi:10.3748/wjg.v31.i20.105554

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. May 28, 2025; 31(20): 105554
Published online May 28, 2025. doi: 10.3748/wjg.v31.i20.105554

Table 2 Antibody characteristics

Protein	Application	Origin	Dilution
α-SMA	WB and IHC-P	ab5694, Abcam, United Kingdom	1:200
RCAN1.4	WB	ab140131, Abcam, United Kingdom	1:10000
CaN	WB	55147-1-AP, Proteintech, China	1:1000
NFAT3	WB and IHC and IF	bs-6461R, Bioss, China	1:1000
β-tubulin	WB and IHC and IF	10094-1-AP, Proteintech, China	1:5000
GAPDH	WB and IHC and IF	ab9485, Abcam, United Kingdom	1:2500
Lamin B1	WB and IHC and IF	12987-1-AP, Proteintech, China	1:5000
HRP-conjugated affinipure goat anti-rabbit IgG (High + Low)	ELISA, WB	SA00001-2, Proteintech, China	1:5000

WB: Western blot; IF: Immunofluorescence; IHC: Immunohistochemistry; ELISA: Enzyme-linked immunosorbent assay; IgG: Immunoglobulin; HRP: Horseradish peroxidase; RCAN: Regulator of calcineurin; α-SMA: α-smooth muscle actin; CaN: Calcineurin; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.

Citation: Lyu SY, Xiao W, Chen YJ, Liao QL, Cai YY, Yu C, Liu JY, Liu H, Zhang MP, Ren YL, Yu QL, Qi YM, Xiao EH, Luo YH. Multi-parameter magnetic resonance imaging of zebularine in liver fibrosis treatment and calcineurin/NFAT3 mechanism. World J Gastroenterol 2025; 31(20): 105554
URL: https://www.wjgnet.com/1007-9327/full/v31/i20/105554.htm
DOI: https://dx.doi.org/10.3748/wjg.v31.i20.105554