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©The Author(s) 2025.
World J Gastroenterol. May 28, 2025; 31(20): 105554
Published online May 28, 2025. doi: 10.3748/wjg.v31.i20.105554
Published online May 28, 2025. doi: 10.3748/wjg.v31.i20.105554
Table 1 Primer sequences for real-time polymerase chain reaction
| Gene (human) | Forward | Reverse |
| CoL1a1 | 5’-AGACGAAGACATCCCACCA-3’ | 5’-GTCGCAGACGCAGATCC-3’ |
| ACTA2 (α-SMA) | 5’-GTTACGAGTTGCCTGATGG-3’ | 5’-AGGTGGTTTCATGGATGC-3’ |
| SEPTIN9 | 5’-GTGAAGAACTCAGAACCCTCG-3 | 5’-TCAATGGACAGCTCAGTGC-3’ |
| PPAR-γ | 5’-AGATCATTTACACAATGCTGGC-3’ | 5’-TAAAGTCACCAAAAGGCTTTCG-3’ |
| PTEN | 5’-GACCAGAGACAAAAAGGGAGTA-3’ | 5’-ACAAACTGAGGATTGCAAGTTC-3’ |
| RCAN1.4 | 5’-TTTAGCTCCCTGATTGCCTGT-3’ | 5’-AAAGGTGATGTCCTTGTCATACG-3’ |
| PTCH1 | 5’-TTTTCTGCTGTTTTACAAGCCC-3’ | 5’-CATGGTAATCTGCGTTTCATGG-3’ |
| β-actin (loading control) | 5’-TCCCTGGAGAAGAGCTACGA-3’ | 5’-TGAAGGTAGTTTCGTGGATGC-3’ |
- Citation: Lyu SY, Xiao W, Chen YJ, Liao QL, Cai YY, Yu C, Liu JY, Liu H, Zhang MP, Ren YL, Yu QL, Qi YM, Xiao EH, Luo YH. Multi-parameter magnetic resonance imaging of zebularine in liver fibrosis treatment and calcineurin/NFAT3 mechanism. World J Gastroenterol 2025; 31(20): 105554
- URL: https://www.wjgnet.com/1007-9327/full/v31/i20/105554.htm
- DOI: https://dx.doi.org/10.3748/wjg.v31.i20.105554