Clinical, genetic and functional perspectives on ATP-binding cassette subfamily B member 4 variants in five cholestasis adults

Figure 3 Immunohistochemical manifestations of the liver in a patient with ATP-binding cassette subfamily B member 4 variants. Except for patient 4, all other patients underwent liver biopsy. Immunohistochemical staining was performed using anti- ATP-binding cassette subfamily B member 4 (P3II-26, Thermo, United States), with the white arrows indicating the labeling of multidrug resistance protein 3 (MDR3) tubules. Bars = 2.5 μm. A: MDR3 is evenly distributed in liver tissue (the white arrow indicates MDR3 is normal, case 1); B: MDR3 is evenly distributed in liver tissue (the white arrow indicates MDR3 is normal, 400 ×, case 2); C: Decreased distribution of MDR3 in liver tissue (the white arrow indicates MDR3 is decreasing, 400 ×, case 3); D: Decreased distribution of MDR3 in liver tissue (the white arrow indicates MDR3 is decreasing, 400 ×, case 5). ABCB4: ATP-binding cassette subfamily B member 4; MDR3: Multidrug resistance protein 3.