Trichostatin A augments cell migration and epithelial-mesenchymal transition in esophageal squamous cell carcinoma through BRD4/c-Myc endoplasmic reticulum-stress pathway

Figure 3 Trichostatin A promotes epithelial-mesenchymal transition of esophageal squamous cell carcinoma cells through cellular myelocytomatosis oncogene mediated endoplasmic reticulum stress. A: Western blot shows after trichostatin A (TSA) treatment that cellular myelocytomatosis oncogene (c-Myc) level increased; B: Relative protein level of c-Myc in control cells and c-Myc-knockdown cells treated with TSA was examined by western blot; C: Representative images of the migration of Eca109 and EC9706 cells knockdown c-Myc protein and treated with TSA examined by Transwell assays; D: Western blot shows after TSA combined with sic-Myc that endoplasmic reticulum stress and epithelial-mesenchymal transition marker E-cadherin level increased, glucose-regulated protein 78, vimentin, β-catenin level decreased. Cell counts are for the corresponding assays of at least five random microscope fields (100 magnification). n = 5, P vs control. ^aP < 0.05. ^bP < 0.01. Bar is 50 μm. TSA: Trichostatin A; GRP78: Glucose-regulated protein 78; c-Myc: Cellular myelocytomatosis oncogene.