SLC6A14 promotes ulcerative colitis progression by facilitating NLRP3 inflammasome-mediated pyroptosis

Figure 3 Downregulating SLC6A14 promotes proliferation and inhibits apoptosis in lipopolysaccharide-treated intestinal epithelial cells. A and B: The expression of SLC6A14 in lipopolysaccharide (LPS)-, LPS+shCTRL-, and LPS+sh-SLC6A14-treated FHC and NCM460 cells was assessed by quantitative real-time polymerase chain reaction (A) and Western blotting (B); C: Cell viability was determined by CCK-8 assays; D and E: An EdU assay was used to detect cell proliferation, and the scale bar represents 100 μm; F and G: Flow cytometry showing apoptosis in FHC and NCM460 cells, followed by quantitative analyses. ^aP < 0.01 vs controls; ^bP < 0.01 vs LPS+sh-CTRL. LPS: Lipopolysaccharide.