SLC6A14 promotes ulcerative colitis progression by facilitating NLRP3 inflammasome-mediated pyroptosis

Figure 2 Induction of pyroptosis by lipopolysaccharide in FHC and NCM460 intestinal epithelial cells. A: CCK-8 assay showing the impact of lipopolysaccharide (LPS) on FHC and NCM460 cell proliferation; B: EdU assay showing the proliferation of LPS-treated and untreated FHC and NCM460 cells; C: Quantification of EdU-positive cells; D and E: Flow cytometric analysis of apoptosis in cells with and without LPS treatment; F and G: Enzyme-linked immunosorbent assay analysis of the effects of LPS on IL-1β and IL-18 secretion; H: Western blot analysis showing the levels of pyroptosis-associated proteins in LPS-treated and untreated cells; I and J: Western blot analysis showing SLC6A14 levels in LPS-treated and control cells. ^aP < 0.01 vs controls. LPS: Lipopolysaccharide.