Fusobacterium nucleatum-induced imbalance in microbiome-derived butyric acid levels promotes the occurrence and development of colorectal cancer

Figure 9 Adenosine monophosphate-activated protein kinaseα-specific small interfering RNA effectively reduced the expression of adenosine monophosphate-activated protein kinase and its phosphorylation in DLD-1 and HCT116 cells. A and B: Adenosine monophosphate-activated protein kinase (AMPK)α-specific small interfering RNA (siRNA) reduces AMPK phosphorylation, increases the expression of proteins such as C-myc, CDK1, and Cyclin B1, and reduces the expression of P21. Data are expressed as mean ± standard deviation, from at least three independent experiments. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. NC: Control; p-AMPK: Phosphorylated-adenosine monophosphate-activated protein kinase.