Silent information regulator sirtuin 1 ameliorates acute liver failure via the p53/glutathione peroxidase 4/gasdermin D axis

Figure 5 Silent information regulator sirtuin 1 interdicts the glutathione peroxidase 4/gasdermin D signaling pathway by intercepting p53 deacetylation. A: Macroscopic examinations of mouse livers and microscopic examinations of liver sections stained with hematoxylin and eosin (100 ×); B: Survival time of mice after resveratrol and EX527 treatments; C: Multi-factor kit for the Detection of interleukin (IL)-1β, tumour necrosis factor alpha, IL-6, IL-2, and C-C motif ligand 2 in mouse serum with multi-factor kit; D and E: Western blot analyses of silent information regulator sirtuin 1, acetylated p53, p53, glutathione peroxidase 4, solute carrier family 7a member 11, and Acyl-CoA synthetase long-chain family member 4 protein expression (n = 3 in each group). ^aP < 0.05, ^bP < 0.01. ALF: Acute liver failure; AST: Aspartate aminotransferase; ALT: Alanine aminotransferase; IL: Interleukin; GPX4: Glutathione peroxidase 4; SLC7A11: Solute carrier family 7a member 11; ACSL4: Acyl-CoA synthetase long-chain family member 4; GSDMD: Gasdermin D; SIRT1: Silent information regulator sirtuin 1; LPS: Lipopolysaccharide; D-GalN: D-galactosamine; RSL3: RAS-selective lethal 3; RSV: Resveratrol.