Exosome-mediated transfer of circRNA563 promoting hepatocellular carcinoma by targeting the microRNA148a-3p/metal-regulatory transcription factor-1 pathway

Figure 5 Oncogenic effect of exosomal circ563 prevented by either miR-148a-3p upregulation or metal-regulatory transcription factor-1 knockdown. A-P: Exosomal circ563 promoted Hep3B cell proliferation and invasion, which were reversed by miR-148a-3p overexpression or metal-regulatory transcription factor-1 depletion. Group 1: Co-culturing Hep3B cells with mesenchymal stem cell (MSC)-derived exosomes; Group 2: Co-culturing Hep3B cells with exosomes derived from circ563-overexpressing MSCs; Group 3: Co-culturing Hep3B cells after miR-148a-3p upregulation with exosomes derived from circ563-overexpressing MSCs; and Group 4: Co-culturing Hep3B cells after metal-regulatory transcription factor-1 knockdown with exosomes derived from circ563-overexpressing MSCs. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001.