Suberoylanilide hydroxamic acid upregulates reticulophagy receptor expression and promotes cell death in hepatocellular carcinoma cells

Figure 1 Impact of suberoylanilide hydroxamic acid treatment on cell viability, cell cycle dispersion, and cellular apoptosis in Huh7 and MHCC97L cells. A: Huh7 and MHCC97L cells were treated with 0, 0.5, 1, 3, 6, 9, 12, 18, and 24 μmol/L suberoylanilide hydroxamic acid (SAHA) for 48 h. The proliferation of Huh7 and MHCC97L cells upon SAHA treatment was monitored using a cell counting kit-8 assay; B-F: Huh7 and MHCC97L cells were exposed to 0, 1, 3, 6, and 12 μmol/L SAHA for 48 h. The proportions of apoptotic cells and the cell cycle dispersion were detected using flow cytometer. Experiments were repeated thrice. Representative results from three independent replicate assays are shown. Data are exhibited as mean ± standard deviation. ^aP < 0.05 vs 0 μmol/L group (n = 3). PI: Propidium iodide.