Diabetes exacerbates inflammatory bowel disease in mice with diet-induced obesity

Figure 6 Colonic mucin barrier and tight junction protein abundance in diet-induced obese mice on antidiabetic treatment with dextran sodium sulfate colitis. A: Alcian blue (AB) staining highlighted the colonic mucin barrier in high-fat diet (HFD)-fed mice administered either vehicle (Veh) or streptozotocin (STZ), followed by antidiabetic treatment with Veh or a sodium-glucose cotransporter-2 inhibitor (SGLT2i) prior to the dextran sodium sulfate (DSS) course; B: AB staining following the DSS course in areas of the colon with intact epithelium was quantified for each treatment group that received Veh/STZ or Veh/SGLT2i; C: Correlation of degree of hyperglycemia with AB staining; D: Tight junction protein E-cadherin staining in the same HFD-fed, DSS-treated mice that received Veh/STZ and Veh/SGLT2i; E: E-cadherin abundance following the DSS course in areas of the colon with intact epithelium; F: Correlation of degree of hyperglycemia with E-cadherin abundance. n = 7-8 per group, mean ± standard error of the mean. DSS: Dextran sodium sulfate; Veh: Vehicle; STZ: Streptozotocin; HFD: High-fat diet; SGLT2i: Sodium-glucose cotransporter-2 inhibitor.