In vivo recognition of bioactive substances of Polygonum multiflorum for protecting mitochondria against metabolic dysfunction-associated fatty liver disease

doi:10.3748/wjg.v29.i1.171

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World Journal of Gastroenterology

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1007-9327

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World J Gastroenterol. Jan 7, 2023; 29(1): 171-189
Published online Jan 7, 2023. doi: 10.3748/wjg.v29.i1.171

Table 2 Ultra-high performance liquid chromatography/mass spectrometry data and chemical structure assignment of Polygonum multiflorum-derived monomers from liver mitochondria

No	T_R (min)	(M-H)- (m/z)	Molecular formula	Predicted (m/z)	Measured (m/z)	Diff (ppm)	ESI-MSn(-), m/z (abundance)	Assigned identification	Ref.
1	18.87	269.0454	C₁₅H₁₀O₅	269.0450	269.0454	3.457	269.04541 (100.00), 241.05089 (2.18), 225.14899 (1.98)	Aloe-emodin¹	[12-14]
2	15.90	431.0981	C₂₁H₂₀O₁₀	431.0978	431.0981	2.011	431.109811 (34.23), 311.05627 (4.32), 269.04553 (100.00), 240.04503 (0.69)	Emodin-8-O-glucoside¹	[14-16]
3	33.91	269.0457	C₁₅H₁₀O₅	269.0450	269.0457	4.461	269.04553 (100), 241.05331 (0.18), 225.05482 (1.37)	Emodin¹	[15-19]
4	18.80	445.0778	C₂₁H₁₈O₁₁	445.0771	445.0778	2.881	269.04553 (100.00), 225.05527 (0.19)	Emodin-glucuronide	[17]
5	9.06	405.1190	C₂₀H₂₂O₉	405.1186	405.1190	2.422	243.06613 (62.27)	2,3,5,4’-Tetrahydroxy stilbene-¹	[17]
6	13.17	433.1136	C₂₁H₂₂O₁₀	433.1135	433.1136	1.539	257.08191 (100.00), 175.02435 (8.79), 152.99582 (3.58), 113.02423 (59.47)	Emodin-8-O-β-D-glucopyranoside reduction metabolite	[17]
7	13.46	245.0820	C₁₄H₁₄O₄	245.0814	245.0820	4.834	245.08208 (87.57), 230.05850 (9.07)	Torachrysone¹	[17]
8	15.15	421.1142	C₂₀H₂₂O₁₀	421.1135	421.1142	3.032	245.08179 (76.39), 230.05852 (4.02)	Torachrysone-glucuronide	[17]

¹Compared with reference substances.

Citation: Yu LP, Li YJ, Wang T, Tao YX, Zhang M, Gu W, Yu J, Yang XX. In vivo recognition of bioactive substances of Polygonum multiflorum for protecting mitochondria against metabolic dysfunction-associated fatty liver disease. World J Gastroenterol 2023; 29(1): 171-189
URL: https://www.wjgnet.com/1007-9327/full/v29/i1/171.htm
DOI: https://dx.doi.org/10.3748/wjg.v29.i1.171