Interleukin-34 deficiency aggravates development of colitis and colitis-associated cancer in mice

Figure 7 Interleukin-34 deficiency promotes azoxymethane /dextran-sodium-sulfate-induced colitis-associated tumorigenesis. A: Experimental design was shown as diagram. WT and Interleukin-34 (IL-34)^-/- mice were given a single injection of azoxymethane (AOM, 10 mg/kg). Seven days later, mice were treated with 1.25% dextran sodium sulfate (DSS) (w/v) in drinking water for seven consecutive days, and given fresh drinking water for 14 d. Seven days of DSS and 14 d of fresh water as a cycle, repeated four times; B: Kaplan-Meier survival curves were plotted in IL-34^-/- and WT mice treated with AOM/DSS (n = 10 per group); C and D: Representative photo of colon (C) and tumor number in colon (D) in IL-34^-/- and WT mice treated with AOM/DSS (n = 10 per group); E: Representative hematoxylin and eosin-stained colon sections in IL-34^-/- and WT mice treated with AOM/DSS (n = 10 per group). AOM: Azoxymethane; DSS: Dextran sodium sulfate; WT: Wild type. Bars: 1 mm (top) and 100 μm (bottom). Data depict mean ± SD. ^aP < 0.05.