Serum metabolic profiling of targeted bile acids reveals potentially novel biomarkers for primary biliary cholangitis and autoimmune hepatitis

Figure 6 Multivariate statistical analysis on serum profiling data in negative ions between primary biliary cholangitis and autoimmune hepatitis. A: Plots of principal component analysis in negative ion mode. (1) Autoimmune hepatitis (AIH); (2) Primary biliary cholangitis (PBC); and (3) Quality control; B: Scatter plots of partial least squares-discriminant analysis (PLS-DA) with a negative model of serum from patients with PBC, AIH and healthy controls. (1) AIH; and (2) PBC; C: Validation plot of the original PLS-DA with a negative model, strongly indicating that the original model is valid and shows signs of overfitting. The permutation test was repeated 200 times in the cross-validation plot.